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目的:探讨8周间歇运动和G-CSF动员对心梗(MI)大鼠心肌血管新生数量及血管再生通路因子VEGF/VEGFR-2的影响。方法:3月龄雄性SD大鼠,体质量185~210 g,结扎LAD建立MI模型。术后存活的大鼠随机分为假手术对照组(A组)、MI组(B组)、间歇运动+MI组(C组)、动员剂+MI组(D组)和间歇运动+动员剂+MI组(E组),每组10只。C组和E组进行8周跑台间歇运动训练,8周后免疫组织化学方法染色测定VEGF、VEGFR-2表达量和CD31表达数量,用血管墨汁灌注法对MI区血管形成情况进行观察。结果:免疫组化结果显示,各干预手段均可上调MI大鼠心肌VEGF、VEGFR-2、CD31表达(数)量,且E组>D组>C组;血管墨汁染色结果显示,各干预手段均可促进MI区血管、血管网的形成。结论:间歇运动和/或G-CSF均显著上调了MI大鼠心肌血管再生通路因子VEGF/VEGFR的表达,促进血管再生,增加血管新生数量,且间歇运动联合G-CSF动员的双重作用效果更佳,可能与2者有效动员内皮祖细胞(EPCs)数量和能力,参与、分化为新生血管有关。该研究为有效地治疗缺血性心脏病提供基础实验依据。
AIM: To investigate the effects of 8-week intermittent exercise and G-CSF mobilization on myocardial angiogenesis and VEGF / VEGFR-2 in myocardial infarction (MI) rats. Methods: Three-month-old male Sprague-Dawley rats with body weight of 185-210 g were infused with LAD to establish MI model. Surviving rats were randomly divided into sham operation control group (group A), MI group (group B), intermittent exercise + MI group (group C), mobilization group + MI group (group D) and intermittent exercise + mobilization + MI group (E group), 10 rats in each group. Group C and group E were subjected to treadmill training for 8 weeks. After 8 weeks, the expression of VEGF and VEGFR-2 and the number of CD31 were detected by immunohistochemical staining. The angiogenesis in MI area was observed by ink instillation. Results: The results of immunohistochemistry showed that all interventions could increase the expression of VEGF, VEGFR-2 and CD31 in myocardium of MI rats, and E> D group> C group. The results of vascular ink staining showed that, Can promote the MI area of blood vessels, vascular network formation. CONCLUSION: Intermittent exercise and / or G-CSF significantly up-regulated the expression of VEGF / VEGFR, and promoted angiogenesis and angiogenesis in MI rats, and the dual effect of intermittent exercise combined with G-CSF mobilization Good, may be 2 and effective mobilization of endothelial progenitor cells (EPCs) the number and ability to participate in differentiation into neovascular. This study provides the basic experimental evidence for the effective treatment of ischemic heart disease.