目的 :探讨不同血清浓度的培养基对颌下腺 (SMG)导管细胞增殖的影响。方法 :应用相差显微镜及台盼蓝染色活细胞计数 ,观察 5 %、10 %、15 %、2 0 %浓度血清在 1～ 11d对SMG导管细胞增殖的影响。结果 :培养2 4～ 3 6h各组间无显著性差异 (P >0 .0 5 ) ,随着时间延长 ,5 %组培养的细胞逐渐死亡 ;至 5d后 ,2 0 %组活细胞数目与其它各组相比较有显著性差异 (P <0 .0 5 )。结论 :SMG导管细胞是一种血清依赖性较高的细胞 Objective: To investigate the effects of different serum concentrations on the proliferation of submandibular gland (SMG) ductal cells. Methods: The number of viable cells was counted by phase contrast microscope and trypan blue staining. The effects of 5%, 10%, 15% and 20% serum on the proliferation of SMG ductal cells were observed at 1 ~ 11 days. Results: There was no significant difference between the two groups (P> 0.05). The cells in 5% group died gradually with time prolonging. After 5 days, the number of living cells in 20% The other groups were significantly different (P <0.05). Conclusion: SMG ductal cells are a highly serum-dependent cell