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为确定以色列不同群体野生大麦的遗传差异,利用42对SSR引物对采自以色列不同地区的9个群体144份野生大麦遗传多样性进行了研究。结果表明,42个SSR标记共检测到189个等位变异,平均每个标记有4.5个,变幅为3~7个。标记多态性信息含量(PIC)平均值为0.696 8,变幅为0.529 9~0.823 3。聚类结果表明,144份材料间的遗传相似系数为0.550 3~0.936 5,平均值为0.701 5。遗传相似(GS)值为0.678 0水平分为5个群体,第Ⅰ类群共43份:包括Shilat地区17份、Modien地区13份和Mehola地区13份;第Ⅱ类群包括15份:包括Nahariya地区11份和Golan High地区4份;第Ⅲ类群共55份:包括Sede Boqer Cangyn地区15份、Sede Boqer Way地区18份和Sede Boqer Highway地区22份;第Ⅳ类群共30份:包括Hermon地区24份和Golan High地区6份;第Ⅴ类群只有Sha 23,分类界限不明显。且中部的群体与南、北部的群体出现了交叉分类;主坐标分析与聚类分析结果大体一致,交叉分类结果更直观。本研究有助于今后发掘优良基因和分子辅助筛选。
To determine the genetic diversity of wild barley from different groups in Israel, 42 pairs of SSR primers were used to study the genetic diversity of 144 wild barley collected from 9 populations in different regions of Israel. The results showed that a total of 189 alleles were detected in 42 SSR markers, with an average of 4.5 markers per marker and amplitude ranging from 3 to 7. The average value of the marker polymorphism information content (PIC) was 0.696 8 and the amplitude was 0.529 9 ~ 0.823 3. The clustering results showed that the genetic similarity coefficients of 144 materials ranged from 0.550 3 to 0.936 5 with an average of 0.701 5. The genetic similarity (GS) value was 0.678 0 divided into 5 groups and the first group consisted of 43 plants: 17 in Shilat, 13 in Modien and 13 in Mehola; Group II in 15, including Nahariya 11 4 in the Golan High area and 55 in the group III including 15 in the Sede Boqer Cangyn area, 18 in the Sede Boqer Way area and 22 in the Sede Boqer Highway area; 30 in the IV group including 24 in the Hermon area and Golan High region 6; Class V only Sha 23, the classification boundaries are not obvious. Cross-classification was found between the central and southern populations and the northern populations. The results of principal-axis and cluster analyzes were generally consistent, and the cross-classification results were more intuitive. This study will help to discover excellent genes and molecular-assisted screening in the future.