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目的探讨副结核杆菌（Ｍｙｃｏｂａｃｔｅｒｉｕｍｐａｒａｔｕｂｅｒｃｕｌｏｓｉｓ，Ｍｐ）在克隆病（Ｃｒｏｈｎ＇ｓｄｉｓｅａｓｅ，ＣＤ）病因学中的作用．方法将５例ＣＤ患者肠道的活检组织制成匀浆，１００μＬ作ＰＣＲ分析，４０μＬ接种地鼠肠壁后４ｍｏ，作肉眼和组织学观察，炎症程度评分按Ｔａｎａｋａ的肠道损伤积分标准．结果５例ＣＤ肠粘膜匀浆中，有２例扩增出Ｍｐ４００ｂｐ的ＤＮＡ片段．用ＰＣＲ阳性组ＣＤ匀浆接种１０只地鼠后，有５只出现了肉芽肿性改变，并再次ＰＣＲ扩增检出了Ｍｐ．ＰＣＲ阳性组肠道损伤平均积分为４８±２４；ＰＣＲ阴性组仅有１只出现肉芽肿性炎症，积分为３７±１７，两组无显著性差异（Ｐ＞００５）；而两组动物肉芽肿性肠炎的发病率有显著性差异（Ｐ＜００５）；ＣＤ匀浆组和正常肠粘膜组的积分有显著性差异（Ｐ＜００５）．结论Ｍｐ确实可能为部分ＣＤ的致病菌．
Objective To investigate the role of Mycobacterium tuberculosis (Mp) in the etiology of Crohn’s disease (CD). Methods The biopsies from 5 CD patients were homogenized and 100 μL were analyzed by PCR. After 40 μL was inoculated into the intestinal wall of the hamster, macroscopic and histological changes were observed. The severity of inflammation was evaluated according to Tanaka’s intestinal injury score. Results In 5 cases of CD intestinal mucosa homogenate, 2 cases amplified Mp400bp DNA fragment. After inoculation of 10 hamsters with CD-positive in PCR-positive group, 5 cases showed granulomatous changes, and Mp was detected by PCR amplification again. The average score of intestinal injury in PCR-positive group was 48 ± 24. Only 1 patient in PCR-negative group showed granulomatous inflammation with an integral score of 37 ± 17, with no significant difference between the two groups (P> 0 05). The incidence of granulomatous enteritis was significantly different between the two groups (P <005). There was significant difference between the CD homogenate group and the normal intestinal mucosa group (P <005). Conclusion Mp may indeed be part of the pathogens of CD.