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目的 探讨Epstein Barr病毒 (EBV)BARF1基因单独或协同佛波醇乙酯 (TPA)诱发猴肾上皮细胞恶性转化的作用。方法 用猴肾永生化上皮细胞PT1和PT7单独或进一步加促癌物TPA注射裸鼠或Scid小鼠背部皮下 ,观察成瘤情况。结果 EB病毒BARF1永生化细胞PT1和PT7在裸鼠不能形成肿瘤 ,但在免疫力严重缺陷的Scid小鼠能形成肿瘤 ,成瘤率为 6 6 7%～ 10 0 % ,成瘤时间较长为45～ 6 0d。加TPA后裸鼠及Scid小鼠均能形成肿瘤 ,裸鼠的成瘤时间为 2 0～ 2 2d ,Scid小鼠的成瘤时间为 5～ 7d。用聚合酶链反应 (PCR)可从肿瘤组织中扩增出BARF1基因。结论 EB病毒BARF1基因是致癌基因 ,甚至无需促癌物等辅助条件 ,也能诱发猴肾上皮细胞恶性转化
Objective To investigate the role of BARF1 gene of Epstein Barr virus (EBV) in the malignant transformation of monkey kidney epithelial cells induced by TPA. Methods The immortalized monkey kidney cells PT1 and PT7 alone or further plus TPA were injected into nude mice or Scid mice subcutaneously on the back to observe tumor formation. Results EBV-BARF1 immortalized cells PT1 and PT7 could not form tumors in nude mice, but Scid mice with severe immunodeficiency could form tumors with a tumorigenic rate of 66.7% -10.0% and longer tumorigenicity 45 ~ 60d. Tumors can be formed in both nude mice and Scid mice after TPA treatment. The time to tumor formation in nude mice was 20 to 22 days and that in Scid mice was 5 to 7 days. The BARF1 gene was amplified from tumor tissue by polymerase chain reaction (PCR). Conclusions The EBV BARF1 gene is an oncogene that can induce malignant transformation of monkey kidney epithelial cells even without the aid of carcinogens and other auxiliary conditions