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目的观察姜黄素对糖尿病大鼠肾脏血管紧张素Ⅰ型(AT1)受体表达的影响。方法选取瘦型及肥胖型Zucker大鼠各16只,随机分为瘦型溶剂(Lean Vehicle)组、肥胖型溶剂(Obese Vehicle)组、瘦型姜黄素(Lean Curcumin)组及肥胖型姜黄素(Obese Curcumin)组。分别给予溶剂或姜黄素(2.0mmol/L)口服喂养4周后,ELISA测定血清丙二醛(MDA)和谷胱甘肽(GSH)水平。RT-PCR及Western blot印迹法测定肾脏AT1受体mRNA及蛋白表达。结果 Obese Vehicle组MDA水平较Lean Vehicle组升高[(3.5±0.8)vs(6.1±0.6)mmol/ml],GSH水平较Lean Vehicle组降低[(7.4±0.7)vs(3.4±0.5)nmol/mg)](P<0.05)。肾脏AT1受体mRNA及蛋白表达亦具有同样的规律(P<0.05)。姜黄素处理后,Obese Curcumin组MDA较Obese Vehicle组降低,GSH较Obese Vehicle组升高(P<0.05),同时AT1受体mRNA及蛋白表达也降低(P<0.05)。结论姜黄素可降低肥胖大鼠氧化应激水平及肾脏AT1受体的表达,促进其血压的降低。
Objective To observe the effects of curcumin on the expression of angiotensin type 1 (AT1) receptor in kidney of diabetic rats. Methods Sixteen lean and obese Zucker rats were randomly divided into Lean Vehicle group, Obese Vehicle group, Lean Curcumin group and obese curcumin group Obese Curcumin) group. Serum MDA and GSH levels were measured by ELISA after four weeks of oral administration of either solvent or curcumin (2.0 mmol / L). The mRNA and protein expression of AT1 receptor in kidney were determined by RT-PCR and Western blotting. Results Compared with Lean Vehicle group, MDA level in Obese Vehicle group was significantly higher than that in Lean Vehicle group [(3.5 ± 0.8) vs (6.1 ± 0.6) mmol / ml] [(7.4 ± 0.7 vs 3.4 ± 0.5) nmol / mg)] (P <0.05). Kidney AT1 receptor mRNA and protein expression also have the same rule (P <0.05). After treatment with curcumin, MDA in Obese Curcumin group was lower than that in Obese Vehicle group, GSH was higher than that in Obese Vehicle group (P <0.05), and mRNA and protein expression of AT1 receptor were also decreased (P <0.05). Conclusion Curcumin can reduce the level of oxidative stress and the expression of AT1 receptor in kidney in obese rats and promote the decrease of blood pressure.