论文部分内容阅读
目的通过检测PTEN基因在宫内发育迟缓(IUGR)大鼠肝脏中的表达,探讨PTEN基因在IUGR致胰岛素抵抗(IR)中的意义。方法通过孕期低蛋白饮食法建立大鼠IUGR模型,检测90 d雄性IUGR大鼠胰岛素敏感性,采用反转录(RT)-PCR技术检测90 dIUGR组及对照组仔鼠肝脏PTEN基因mRNA表达水平,Western blot检测肝脏组织中丝氨酸/苏氨酸激酶(AKT)蛋白及磷酸化AKT蛋白表达水平。采用SPSS 13.0软件进行统计学处理。结果与对照组比较,IUGR雄性仔鼠平均出生体质量显著降低(t=14.73,P<0.05),空腹血糖显著升高(t=-3.11,P=0.011),空腹胰岛素显著升高(t=-5.01,P=0.001),胰岛素抵抗指数显著升高(t=-3.06,P=0.013),胰岛素敏感指数显著降低(t=2.80,P=0.019),PTEN基因mRNA表达显著升高(t=-2.40,P=0.04),AKT蛋白表达虽然无统计学差异,但磷酸化AKT蛋白表达显著下降(t=3.02,P=0.01),PTEN与胰岛素抵抗指数呈正相关(r=0.928,P<0.05),与磷酸化AKT的表达呈负相关(r=-0.411,P<0.05)。结论 PTEN可能是IUGR致IR的重要调节分子之一。
Objective To investigate the significance of PTEN gene in insulin resistance (IR) induced by IUGR by detecting the expression of PTEN gene in the liver of intrauterine growth retardation (IUGR) rats. Methods Rat IUGR model was established by low protein diet during pregnancy. Insulin sensitivity in 90-day male IUGR rats was detected. MRNA expression of PTEN gene in liver of 90 dIUGR group and control group was detected by reverse transcription-polymerase chain reaction (RT-PCR) Western blot was used to detect the expression of serine / threonine kinase (AKT) protein and phosphorylated AKT protein in liver tissues. Using SPSS 13.0 software for statistical analysis. Results Compared with the control group, the average birth weight of IUGR male offspring rats was significantly lower (t = 14.73, P <0.05) and fasting blood glucose was significantly higher (t = -3.11, P = 0.011) (T = -3.06, P = 0.013), insulin sensitivity index (t = 2.80, P = 0.001), PTEN mRNA expression was significantly higher (t = -2.40, P = 0.04). Although AKT protein expression was not significantly different, phosphorylated AKT protein expression was significantly decreased (t = 3.02, P = 0.01) and PTEN was positively correlated with insulin resistance index ), Negatively correlated with phosphorylated AKT (r = -0.411, P <0.05). Conclusions PTEN may be one of the important regulators of IR induced by IUGR.