论文部分内容阅读
目的:观察胚胎心肌细胞移植物在大鼠冷冻心肌疤痕中存活的可行性。方法:以冷冻损伤造成16只大鼠左室游离壁心肌疤痕。于损伤后10天,将培养的胚胎心肌细胞或培养基注入心肌疤痕组织,5只动物冷冻后20天处死(疤痕对照组),细胞移植组7只,培养基对照组4只,移植后10天处死动物,取出心脏。结果:疤痕组和培养基对照组动物左室壁损伤局部组织变薄,细胞移植组室壁依然厚实。横纹肌α-肌动蛋白免疫组化染色证实,细胞移植组3只动物被证实有移植物存活。HE染色示移植物呈球形生长,移植细胞体积小,呈梭形,胞核数目多,核/质比例高,横纹不明显,排列不规则。移植物中及其周围未见淋巴细胞浸润。培养基对照组未见任何移植物生长征象。结论:胚胎心肌细胞移植在异体心肌疤痕中能够存活,该实验方法为修复心肌梗死疤痕,改善心脏功能提供了新的治疗策略。
OBJECTIVE: To observe the feasibility of embryonic cardiomyocyte transplantation in the survival of frozen myocardial scar in rats. Methods: Frozen injury caused left ventricular free wall myocardial scar in 16 rats. Ten days after injury, cultured embryonic cardiomyocytes or medium were infused into myocardial scar tissue. Five animals were killed 20 days after freezing (scar control group), 7 in the cell transplantation group, 4 in the control group, 10 in the control group Days dead animals, remove the heart. RESULTS: Local tissues in left ventricular wall of scar group and culture group control group were thinner and the wall of cell transplantation group was still thick. Striated α-actin immunohistochemical staining confirmed that three animals in the cell transplantation group were confirmed to have graft survival. HE staining showed that the grafts grew in a spherical shape. The transplanted cells were small in size and spindle-shaped with many nuclei, high nuclear / cytoplasm proportion, inconspicuous transverse stripes and irregular arrangement. No infiltration of lymphocytes in and around the graft. Media control group showed no signs of graft growth. CONCLUSION: Embryonic cardiomyocyte transplantation can survive in allogeneic myocardial scar. This experimental method provides a new therapeutic strategy for repairing myocardial infarction scar and improving cardiac function.