目的:观察小儿止泻灵对实验性腹泻型、脾虚模型小鼠的治疗作用及对脾虚证小鼠模型肠道菌群的微生态调节作用,为临床使用该药提供科学依据。方法:以苦寒泻下中药制造番泻叶腹泻模型和大黄小鼠脾虚模型,然后给予小儿止泻灵32 g、16 g、8 g生药/kg,观察小儿止泻灵止泻、免疫增强及对脾虚动物肠道厌氧菌的影响。结果:小儿止泻灵对由番泻叶所致小鼠腹泻有明显的抑制作用,对该模型小鼠小肠的推动功能也有明显的抑制作用;对由大黄所致脾虚型小鼠的体重、胸腺系数有明显的增加作用,并能延长该模型小鼠的游泳时间。脾虚证小鼠肠道双歧杆菌、乳杆菌等厌氧菌含量显著下降。应用小儿止泻灵治疗后上述两种厌氧菌含量均恢复正常,且双歧杆菌明显超过造模前水平。结论:小儿止泻灵对小鼠实验性腹泻及脾虚有明显的治疗作用,脾虚证小鼠肠道厌氧菌群异常低,小儿止泻灵具有扶植厌氧菌之调整功能,尤其是通过扶植健康因子双歧杆菌,其治疗脾虚泄泻的作用可能与止泻、增强机体免疫功能、调整肠道菌群作用有关。 OBJECTIVE: To observe the therapeutic effect of Xiaoerzhi Zhiling on experimental diarrhea model and spleen deficiency model mice and the microecological regulation of intestinal microflora in mice model of spleen deficiency syndrome, so as to provide a scientific basis for the clinical use of this medicine. Methods: The model of senna diarrhea and rhubarb mouse model of spleen deficiency were made with bitter cold diarrhea Chinese traditional medicine. Then the children were given Zhixieling 32 g, 16 g, 8 g crude drug / kg. Effect of intestinal anaerobic bacteria in spleen asthenic animals. Results: Xiaozhi Zhixieling inhibited the diarrhea of ​​mice induced by senna obviously and had a significant inhibitory effect on the promoting function of the small intestine of the model mice. The body weight, thymus Coefficient significantly increased the role, and can extend the model of mice swimming time. Spleen deficiency mice intestinal bifidobacteria, Lactobacillus and other anaerobic bacteria decreased significantly. The application of pediatric Zhixie Ling treatment of the above two anaerobic bacteria content returned to normal, and bifidobacteria significantly higher than the pre-model levels. Conclusion: Xiaorenzhixieling has obvious therapeutic effect on experimental diarrhea and spleen deficiency in mice. The anaerobic intestinal flora of mice with spleen deficiency syndrome is abnormally low. Pediatric antidiarrheal spirit can regulate the anaerobic bacteria, especially, Bifidobacterium health factors, its role in the treatment of spleen diarrhea may be diarrhea, enhance immune function, regulating the role of intestinal flora.