论文部分内容阅读
目的 Dukes′B期大肠癌患者手术后 5年生存率为 5 3.9%～ 84.9% ,有部分患者 5年内出现局部复发或 /和远处转移。本研究旨在探讨淋巴结微小转移灶 (lymphnodesmicrometastasisLMM)及原发灶p5 3的表达对评估经手术切除原发肿瘤及肠周淋巴结的Dukes′B期大肠癌患者预后的重要性和指导术后治疗的意义。方法 收集 5 2例Dukes′B期大肠癌患者手术切除的淋巴结及原发灶石蜡标本 ,同时获得这些病例的临床资料及随访 (生存期大于五年或死亡 )资料 ;实验方法 ;以细胞角蛋白单抗 (anti cytokerratinAE1/AE3 )为探针 ,采用免疫组化SAP(streptavidin alkalinephosphatase)法检测淋巴结中的LMM。以p5 3单抗为探针 ,采用免疫组化S -P(streptavidin peroxidase)法检测原发灶p5 3蛋白的表达。结果 检测 5 2例Dukes′B期大肠癌患者的 5年生存率为 73% ( 38/5 2 )。淋巴结中LMM +组 5年生存率 45 .5 % ( 5 /11) ,LMM 组 78.9% ( 32 /41)。原发灶中p5 3+组 5年生存率 6 2 % ( 18/2 9) ,p5 3 组 87% ( 2 0 /2 3)。同时检测LMM +p5 3+组 33% ,LMM p5 3 组 84%。X2 检验均P <0 .0 5 ,生存分析log rank检验P <0 .0 5。本实验中一般因素 (性别、年龄及肿瘤所在部位 )对检测结果的影响不明显 ;统计学处理 ,P >0 .0 5。结论 用
Objective To investigate the 5-year survival rate of patients with Dukes’B colorectal cancer after operation from 5 3.9% to 84.9%. Some patients experienced local recurrence and / or distant metastasis within 5 years. This study aimed to investigate the expression of lymph node micrometastases (lymphnodes microtitrastasisLMM) and primary tumor p5 3 in assessing the prognosis of patients with Dukes’B stage colorectal cancer after primary resection of primary tumors and peri-nal lymph nodes and to guide the postoperative treatment significance. Methods Fifty-two patients with Dukes’B stage colorectal cancer underwent surgical resection of lymph nodes and paraffin-embedded specimens of primary lung cancer. Clinical data and follow-up (survival> 5 years or death) data of these cases were also obtained. Experimental methods; cytokeratin The monoclonal antibody (anti cytokerratin AE1 / AE3) was used as a probe to detect LMM in lymph nodes by streptavidin alkaline phosphatase (SAP). The p5 3 protein was detected by streptavidin peroxidase (S-P) using p5 3 as a probe. Results The 5-year survival rate of 52 patients with Dukes’B colorectal cancer was 73% (38/5 2). The 5-year survival rate was 45.5% (5/11) in the LMM + group and 78.9% (32/41) in the LMM group. The 5-year survival rate of p5 3+ group was 62% (18/2 9) in the primary tumor and 87% (2 0/2 3) in the p5 3 group. At the same time, 33% of LMM + p5 3+ group and 84% of LMM p5 3 group were detected simultaneously. X2 test P <0. 05, survival analysis log rank test P <0. In this experiment, the general factors (gender, age and tumor site) had no significant effect on the test results; Statistical analysis, P> 0.05. Conclusion used