论文部分内容阅读
目的探讨鹧鸪禽流感病毒和人流感病毒唾液酸受体分布特点及其作用。方法用亲和组化法检测鹧鸪呼吸道与消化道流感病毒唾液酸受体的分布,荧光素Alexa488标记禽流感病毒H9N1和人流感病毒H1N1,分别与鹧鸪呼吸道、消化道各解剖部位组织细胞结合。结果鹧鸪呼吸道同时表达SAα-2,3Gal和SAα-2,6Gal两种受体,但其消化道仅表达SAα-2,3Gal受体。SAα-2,3Gal受体在鹧鸪呼吸道各部位和消化道结肠上皮细胞呈强阳性弥漫分布;SAα-2,6Gal受体在呼吸道气管、支气管和次级支气管呈强阳性弥漫分布,而副支气管、消化道结肠均为缺乏或仅表达极少量。H9N1禽流感病毒与鹧鸪呼吸道各部位和消化道结肠均结合;人流感病毒H1N1与鹧鸪的气管、支气管、次级支气管结合,但未见与副支气管和结肠结合,此结果与鹧鸪呼吸道和消化道SAα-2,3Gal和SAα-2,6Gal受体分布总体一致。结论鹧鸪同时表达SAα-2,3Gal和SAα-2,6Gal受体,能与禽流感病毒和人流感病毒结合,有助于禽流感病毒与人流感病毒基因重配,提示其可充当流感病毒潜在的中间宿主。
Objective To investigate the distribution and function of sialic acid receptors in partridge bird flu virus and human influenza virus. Methods The distribution of sialic acid receptors of Partridge’s respiratory tract and gastrointestinal influenza virus were detected by affinity assay. Fluorescein Alexa488 labeled H9N1 and human influenza virus H1N1 were respectively used in combination with the respiratory tract of partridge, the tissue cells of various anatomical parts of digestive tract Combined. Results The partridge respiratory tract expressed both SAα-2, 3Gal and SAα-2, 6Gal receptors, but its digestive tract only expressed SAα-2,3Gal receptors. SAα-2 and 3Gal receptors were strongly positive diffusely distributed in various parts of the respiratory tract and digestive colon epithelial cells. SAα-2 and 6Gal receptors were strongly positive diffusely distributed in the trachea, bronchus and bronchus of the respiratory tract, , Gastrointestinal colon are lacking or only a very small amount of expression. H9N1 bird flu virus is associated with all parts of the respiratory tract of the partridge and the colon of the digestive tract. The human influenza virus H1N1 is associated with the trachea, bronchus and bronchus of the partridge, but not with the bronchus and colon, And gastrointestinal SAα-2,3Gal and SAα-2,6Gal receptor distribution in general. Conclusions Partridge also expressed SAα-2, 3Gal and SAα-2, 6Gal receptors, which can bind to avian influenza virus and human influenza virus and contribute to genetic reassortment of avian influenza virus and human influenza virus, suggesting that it may act as influenza virus Potential intermediate host.