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在实验室条件下研究了浓度分别为0、0.1、0.2、0.4、0.8、1.6和3.2 mg/L胜利原油暴露1、2、4、8、15 d以及解除污染1 d和3 d后对半滑舌鳎幼鱼肝脏过氧化物酶(POD)、溶菌酶(LSZ)和鳃丝Na+-K+-ATPase活力的影响。结果表明:在曝油15 d内,(1)原油浓度0.1、0.2、0.4和0.8 mg/L组肝脏POD活力呈峰值变化,先增大后减小,第8 d时升高到最大值,而原油浓度1.6和3.2 mg/L组肝脏POD活力先减小后增大,第8 d时降低至最小值。(2)原油浓度3.2 mg/L组肝脏LSZ活力先减小后增大,第8 d时降低至最小值,其它实验组肝脏LSZ活力先增大后减小,第2 d时达到最大值,第4 d后趋于稳定。(3)原油浓度0.1 mg/L组鳃丝Na+-K+-ATPase活力与对照组无显著差异(P>0.05),原油高浓度组3.2 mg/L组鳃丝Na+-K+-ATPase活力呈波动变化,其他各实验组鳃丝Na+-K+-ATPase活力先减小后增大,各组均在第1 d降低至最小值。另外恢复3 d后各实验组3种酶活力都能恢复至初始水平,与对照组无显著差异(P>0.05)。因此,3种酶相结合有望成为石油类污染的生物标志物。
Under laboratory conditions, the effects of 1, 2, 4, 8 and 15 days of exposure to Volatile Crude oil of 0, 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2 mg / Effects of POD, LSZ and Na + -K + -ATPase activity in juvenile fish. The results showed that: (1) POD activity in the liver of 0.1,0.2,0.4 and 0.8 mg / L crude oil showed a peak change within 15 days of oil exposure, increasing first and then decreasing, reaching the maximum on the 8th day, However, the POD activity of the liver decreased firstly and then increased at 1.6 and 3.2 mg / L of crude oil, but decreased to the minimum on the 8th day. (2) The LSZ activity of liver decreased firstly and then increased at the crude oil concentration of 3.2 mg / L, and decreased to the minimum value on the 8th day. In other experimental groups, the activity of LSZ first increased then decreased and reached the maximum on the second day, After the 4th d tends to be stable. (3) The Na + -K + -ATPase activity of gill filaments in crude oil concentration of 0.1 mg / L had no significant difference with that in control group (P> 0.05). The Na + -K + -ATPase activity of gill filaments fluctuated in 3.2 mg / L crude oil , And the activity of Na + -K + -ATPase in gill filaments of other experimental groups decreased first and then increased, and each group decreased to the minimum on the first day. In addition, after 3 days of recovery, the activity of three kinds of enzymes in each experimental group recovered to the initial level, no significant difference with the control group (P> 0.05). Therefore, the combination of the three enzymes is expected to become a biomarker of petroleum pollution.