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目的：研究小鼠脾脏是否存在褪黑素（Ｍ）结合位点，该结合位点是否存在昼夜节律。方法：应用放射配体结合法检测小鼠脾脏Ｍ结合位点（ＭＲ）。每４ｈ处死一批动物，研究不同时间脾脏ＭＲ的差异；分别持续光照及注射Ｍ２１ｄ，观察ＭＲ结合量及昼夜节律的改变。结果：小鼠脾脏存在ＭＲ，其特点为低结合容量，高亲和力，对Ｍ具高度特异性，符合特异结合位点的基本条件；昼夜节律研究显示，ＭＲ结合量峰值在１２：００～１６：００，谷值在０：００～４：００，Ｆ检验峰谷值间差异显著（Ｐ＜０．０１）。结论：小鼠脾脏存在ＭＲ，其ＭＲ有昼夜节律变化；持续光照及注射Ｍ不影响ＭＲ节律，表明ＭＲ昼夜节律的机制是自主的，具生理学意义。
Objective: To investigate whether there is melatonin (M) binding site in spleen of mice and whether there is circadian rhythm in the binding sites. Methods: The spleen M binding sites (MRs) of mice were detected by radioligand binding assay. A group of animals were sacrificed every 4 hours to study the difference of spleen MR at different time. The light intensity and injection of M21d were observed respectively, and the changes of MR binding capacity and circadian rhythm were observed. Results: There was MR in the spleen of mice, which was characterized by low binding capacity, high affinity and high specificity for M, which met the basic conditions of specific binding sites. The circadian rhythm study showed that the peak of MR binding was between 12: 00-16: 00, trough in 0: 00 ~ 4: 00, F-test peak-valley difference was significant (P <0.01). CONCLUSION: There is MR in spleen of mice with circadian rhythm of MR. Persistent light and injection of M do not affect MR rhythm, indicating that circadian rhythm of MR is independent and physiologically significant.