1案例资料1.1简要案情作者在对1例非正常死亡案件死者的血样进行DNA检验中,发现D3S1358基因座呈三带型。1.2检验方法STR分型按照硅珠法[1]提取血样模板DNA,用Identifiler Plus荧光检测试剂盒进行PCR扩增,产物在ABI 3130XL遗传分析仪进行分型检测,使用GeneMapper ID-X软件进行数据分析。筛选阳性克隆设计的引物序列见表1。PCR 1 Case Information 1.1 Brief Case Study In a DNA test of blood samples from 1 deceased non-normal death case, the D3S1358 locus showed a three-band type. 1.2 Test methods STR typing according to the method of silica beads [1] extracted blood template DNA, with Identifiler Plus fluorescence detection kit for PCR amplification, the product was ABI 3130XL genetic analyzer type detection, the use of GeneMapper ID-X software for data analysis. Primer sequences for screening positive clones are shown in Table 1. PCR