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目的研究结核蛋白芯片技术、ELISA法检测结核抗体对诊断结核病的价值。方法选取141例结核病患者、52例非结核病患者进行结核蛋白芯片、ELISA法检测结核抗体的表达。结果结核蛋白芯片检测在结核病组表达的阳性率为52.48%,在非结核病组的阳性率为13.46%,差别有统计学意义(P<0.05);ELISA检测在结核病组的阳性率为48.23%,在非结核病组的阳性率为21.15%,两组表达差异有统计学意义(P<0.05)。两种方法联合检测阳性率为81.56%。结论两种检测方法对结核病的诊断均有一定价值,在肺结核患者中检测的阳性率高于非肺结核患者。两种方法联合检测能提高检测的阳性率、敏感度。
Objective To study the technology of tuberculin protein chip and to detect the value of tuberculosis antibody in diagnosis of tuberculosis by ELISA. Methods Totally 141 cases of tuberculosis patients and 52 cases of non-tuberculosis patients were selected for tuberculosis protein chip. ELISA was used to detect the expression of tuberculosis antibody. Results The positive rate of tuberculosis protein chip detection was 52.48% in tuberculosis group and 13.46% in non-tuberculosis group, the difference was statistically significant (P <0.05). The positive rate of tuberculosis protein in tuberculosis group was 48.23% In non-tuberculosis group, the positive rate was 21.15%, the difference between the two groups was statistically significant (P <0.05). The positive rate of the two methods was 81.56%. Conclusion Both methods have some value in the diagnosis of tuberculosis, and the positive rate in tuberculosis patients is higher than that in non-tuberculosis patients. Joint detection of the two methods can improve the detection rate and sensitivity.