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为研究甘蓝型油菜种子fad2基因下调表达对油菜籽营养品质的影响,分析比较了转基因高油酸油菜品系W-4和非转基因对照Westar种子中的脂肪酸、氨基酸组成,以及油份、蛋白质、纤维素和硫甙葡萄糖甙含量等品质指标。数据显示W-4种子中油酸含量达到86.03%±0.20%,较对照增加29.36%,达极显著水平;亚油酸和亚麻酸含量分别为2.86%±0.01%和3.04%±0.04%,较对照分别下降84.03%和57.60%,降幅达极显著水平;棕榈酸含量为3.23%±0.07%较对照Westar降低了18.63%,达极显著水平,而硬脂酸含量差异不显著;廿碳烯酸和芥酸分别较对照增加18.46%和13.15%,达极显著和显著水平;氨基酸组成分析结果显示,W-4和对照种子中均可检测到包括8种必需氨基酸在内的18种氨基酸,除酪氨酸含量低于对照外,其余的差异均不显著;而W-4含油量、蛋白质、硫甙葡萄糖甙和纤维素含量分别为45.40%±0.17%、18.18%±0.91%、18.20%±1.21%和12.29%±0.04%,与对照差异不显著;结果表明油菜种子中fad2基因下调表达对种子的脂肪酸合成与积累影响最大,而对种子油份含量、蛋白质含量、纤维素含量以及硫甙葡萄糖甙的含量无显著影响。
In order to study the effect of down-regulated expression of fad2 gene in rapeseed (Brassica napus L.) seeds on nutritional quality of rapeseed, the fatty acid and amino acid composition in W-4 transgenic and non-transgenic control Westar seeds were compared and analyzed. Sugan and glucosinolate glucoside content and other quality indicators. The data showed that the content of oleic acid in W-4 seeds reached 86.03% ± 0.20%, increased by 29.36% compared with the control, reaching the extremely significant level. The contents of linoleic acid and linolenic acid were 2.86% ± 0.01% and 3.04% ± 0.04% Decreased by 84.03% and 57.60%, respectively; the content of palmitic acid was 3.23% ± 0.07%, which was 18.63% lower than that of control, which reached the extremely significant level, while the content of stearic acid was not significantly different. Erucic acid increased by 18.46% and 13.15%, respectively, compared with the control, reaching extremely significant and significant levels; amino acid composition analysis showed that 18 kinds of amino acids including 8 kinds of essential amino acids were detected in both W-4 and control seeds, While the contents of W-4, glucosinolate, glucosinolate and cellulose in W-4 were 45.40% ± 0.17%, 18.18% ± 0.91%, 18.20% ± 1.21 % And 12.29% ± 0.04%, respectively. The results showed that down-regulation of fad2 gene in rape seeds had the greatest effect on the fatty acid synthesis and accumulation of seeds, but had no significant effect on seed oil content, protein content, cellulose content and glucosinolate glucose Glycoside content had no significant effect.