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目的建立九味肝泰胶囊中人参皂苷Rg1及三七皂苷R1的含量测定方法,弥补该品种质量标准的不完善之处。方法采用ACQUITY BEH C18(2.1 mm×50 mm,1.7μm)色谱柱;流动相为乙腈-水,梯度洗脱;流速为0.4 ml/min;检测波长为203 nm;柱温为35℃;进样体积为1μl。结果人参皂苷Rg1及三七皂苷R1分别在0.10~1.50 mg/ml,0.04~0.60 mg/ml浓度范围内线性良好,平均回收率分别为99.4%、101.8%,RSD分别为0.5%、1.3%。结论该法方便、快速、准确,适用于九味肝泰胶囊中人参皂苷Rg1及三七皂苷R1的含量测定,可用于该制剂的质量控制。
Objective To establish a method for the determination of ginsenoside Rg1 and notoginsenoside R1 in Jiuwei Gengtai Capsule to make up for the imperfection of the quality standard of this variety. Methods The chromatographic column was ACQUITY BEH C18 (2.1 mm × 50 mm, 1.7 μm). The mobile phase consisted of acetonitrile-water with gradient elution. The flow rate was 0.4 ml / min. The detection wavelength was 203 nm. The column temperature was 35 ℃. The volume is 1 μl. Results The concentrations of ginsenoside Rg1 and notoginsenoside R1 were linear in the range of 0.10-1.50 mg / ml and 0.04-0.60 mg / ml, respectively. The average recoveries were 99.4% and 101.8%, respectively. The RSDs were 0.5% and 1.3%, respectively. Conclusion The method is convenient, rapid and accurate and can be applied to the determination of ginsenoside Rg1 and notoginsenoside R1 in Jiuwei Gengtai Capsules. It can be used for the quality control of this preparation.