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Aim:To investigate effects ofCpG oligod’eoxynucleotide(CpG ODN)on the mRNAexpression of transcription factors GATA binding protein 3(GATA-3)and T-boxexpressed in T cells(T-bet)in asthmatic mice.Methods:An asthmatic mousemodel was established and treated with CpG ODN.Total inflammatory cells andeosinophils in bronchoalveolar lavage fluid(BALF)were counted and inflamma-tory cell infiltration in lung tissue was evaluated.Interferon-y and interleukin-4concentrations in BALF and splenocyte culture supernatants were detected us-ing an enzyme-linked immunosorbent assay.Transcription factor GATA-3 and T-bet mRNA expression in splenocytes and lung tissue were detected by reversetranscription-polymerase chain reaction.Results:Total inflammatory cells andeosinophils in BALF were reduced in the CpG ODN-treated group compared withthe asthma group,and inflammatory cell infiltration in lung tissue was also signifi-cantly alleviated.CpG ODN treatment increased the interferon-γ concentrationbut decreased the interleukin-4 concentration in both BALF and splenocyte cul-ture supernatants.GATA-3 mRNA expression was reduced in both lung tissueand splenocytes in the CpG ODN-treated group,while the mRNA ratio ofT-bet toGATA-3 in splenocytes was increased.Conclusion:CpG ODN treatment inhibitsairway inflammatory cell infiltration and regulates interferon-y/interleukin-4 syn-thesis in asthmatic mice,possibly through a mechanism of downregulation ofGATA-3 mRNA expression in both lung tissue and splenocytes.
Aim: To investigate effects of CpG ODN on the mRNA expression of transcription factors GATA binding protein 3 (GATA-3) and T-box expressed in T cells (T-bet) in asthmatic mice. Methods: An asthmatic mouse model was established and treated with CpG ODN. Total inflammatory cells andeosinophils in bronchoalveolar lavage fluid (BALF) were counted and inflamma-tory cell infiltration in lung tissue was performed. Interferon-y and interleukin-4 concentrations in BALF and splenocyte culture supernatants were detected us-ing an enzyme-linked immunosorbent assay. Transcription factor GATA-3 and T-bet mRNA expression in splenocytes and lung tissue were detected by reversetranscription-polymerase chain reaction. Results: Total inflammatory cells andeosinophils in BALF were reduced in the CpG ODN-treated group with the asthma group, and inflammatory cell infiltration in lung tissue was also signifi-cantly alleviated. CpG ODN treatment increased the interferon-gamma concentration but decrea sed the interleukin-4 concentration in both BALF and splenocyte cul-ture supernatants. GATA-3 mRNA expression was reduced in both lung tissue and splenocytes in the CpG ODN-treated group, while the mRNA ratio of T-bet to GATA-3 in splenocytes was increased .Conclusion: CpG ODN treatment inhibits airway inflammatory cell infiltration and regulates interferon-y / interleukin-4 syn-thesis in asthmatic mice, possibly through a mechanism of downregulation of GATA-3 mRNA expression in both lung tissue and splenocytes.