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流感是一种由流感病毒引起的传染病,发病率高、破坏性强,全球每年有25~50万死于流感[1]。目前流感的预防均选择灭活疫苗,但其不能够有效应对流感病毒变异的速度,开发新型疫苗显的尤为重要。多表位核酸疫苗是将抗原基因内部抗原的活性部位与表达质粒通过串联插入而构建,能够有效发挥疫苗内部抗原表位的优势,去除保护性免疫中不利因素(潜在片段中毒性成分结构),同时能够对流感病毒中抗原表位的各种型别进行优化组合,为
Influenza is a contagious disease caused by the influenza virus. It has a high incidence and destructiveness. Each year, 25 to 50 million people die of influenza in the world [1]. At present, influenza prevention is the choice of inactivated vaccine, but it can not effectively deal with the speed of influenza virus mutation, the development of new vaccines was significantly important. Multi-epitope nucleic acid vaccine is constructed by inserting the active site of the internal antigen of the antigen gene and the expression plasmid through the tandem insertion, which can effectively exert the advantages of the internal antigen epitope of the vaccine and remove the unfavorable factors (structures of the toxic components in the latent fragment) At the same time, the various combinations of antigenic epitopes in the influenza virus can be optimized