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B.P.1973.规定市售 Septrin 片中甲氧苄胺嘧啶(Ⅰ)和磺胺甲基异(口恶)唑(Ⅱ)的含量测定,需要事先将药物分离后再测定。现介绍一种不经事先分离,简易、快速的分光光度法。纯样品Ⅰ和Ⅱ由 Burroghs Wellcome &Co.(London)提供;Septrin 片(Burrogh-Wellcome & Co.,London)当地购买;盐酸 N—(1—萘基)乙二胺由 E.Merck(Darm-tadt)提供。配制Ⅰ和Ⅱ的0.1MHCl 溶液,并分别绘制紫外吸收光谱。图1所示,Ⅰ和Ⅱ的0.1MHCl溶液的吸收光谱没有明显不同,而在 pH7.2的溶液中,在240.5nm 处有一个等吸收点。因此,
B.P. 1973. The requirement for the determination of trimethoprim (I) and sulfamethoxazole (II) in commercially available Septrin tablets requires prior determination of the drug after separation. Is introduced without prior separation, simple and rapid spectrophotometry. Pure Samples I and II were supplied by Burroghs Wellcome & Co. (London); Septrin® tablets (Burrogh-Wellcome & Co., London) were purchased locally; N- (1- naphthyl) ethylenediamine hydrochloride was purchased from E. Merck )provide. Preparation Ⅰ and Ⅱ 0.1M HCl solution, and were drawn UV absorption spectra. As shown in Fig. 1, the absorption spectra of 0.1 M HCl solution of I and II did not differ significantly, whereas at pH 7.2 the solution had an isoabsorption point at 240.5 nm. therefore,