论文部分内容阅读
[目的]研究甲状腺癌组织与癌旁正常组织OXTR基因甲基化状态并进行比较,初步探讨OXTR基因甲基化修饰与其表达之间的关系。[方法]利用甲基化特异性PCR(MSP)技术扩增甲状腺癌患者癌组织与癌旁组织DNA甲基化(M)和非甲基化(U)OXTR基因各17例,比较两组甲基化程度差异。[结果]MSP实验结果显示,癌组织(病例组17例)中有11例呈现OXTR基因甲基化条带扩增,其余为OXTR非甲基化条带扩增,甲基化率达到64.7%;癌旁组织(对照组17例)有4例呈现OXTR基因甲基化条带扩增,13例样品皆为OXTR非甲基化条带扩增,对照组OXTR甲基化率为23.5%;两组甲基化程度差异有统计学意义(P<0.05)。[结论]OXTR基因DNA甲基化修饰程度癌组织高于癌旁组织,结合前期血液OXTR基因甲基化修饰研究,该基因甲基化可能是早期肿瘤标志,其检测结果可作为辅助诊断甲状腺癌的有效方法。
[Objective] To investigate the methylation status of OXTR gene in thyroid cancer tissues and adjacent normal tissues, and to explore the relationship between OXTR gene methylation modification and its expression. [Method] Methylation-specific PCR (MSP) was used to amplify DNA methylation (M) and unmethylated (U) OXTR gene in 17 cases of thyroid cancer tissues and adjacent tissues respectively. Differences in the degree of [Results] The results of MSP showed that the methylation of OXTR gene was amplified in 11 cases of cancerous tissue (case group of 17 cases), the rest was OXTR unmethylated band amplification, the methylation rate reached 64.7% ; 4 cases of paracancerous tissues (control group, 17 cases) showed OXTR gene methylation band amplification, 13 cases were OXTR unmethylated bands amplification, the control group OXTR methylation rate was 23.5%; There was significant difference between the two groups in the degree of methylation (P <0.05). [Conclusion] The degree of DNA methylation modification in OXTR gene is higher than that in paracancerous tissues. Combined with methylation modification of OXTR gene in blood, the methylation of OXTR gene may be an early tumor marker. The detection results can be used as an auxiliary diagnosis of thyroid cancer Effective method.