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目的通过分析癌变白斑和稳定白斑病损组织中miRNA表达谱的异常,以期发现可准确评估此类病损癌变风险的生物学标志物。方法使用Taq Man低密度miRNA芯片对稳定白斑和癌变白斑进行分子生物学检测分析。结果同稳定白斑相比较,癌变白斑中miRNAs的表达发生了显著改变,差异有统计学意义(P<0.05),其中13个miRNAs的表达下调(miR-99a-5p、miR-99b-5p、miR-144、miR-100-5p、miR-125-5p、miR-181b、miR-181a、miR-521-3p、miR-339-5p、miR-15a-5p、miR-150-5p、let-7a-5p、miR-9),12个miRNAs的表达上调(miR-10b-5p、miR-708、miR-30e-3p、miR-30a-3p、miR-21、miR-335-5p、miR-144、miR-25-3p、miR-19a-3p、miR-660-5p、miR-140-5p、miR-590-5p)。结论 miRNA生物标志物的检测对稳定白斑和癌变白斑病变的诊断具有重要的意义。
OBJECTIVE: To identify abnormalities of miRNA expression profiles in cancerous leukoplakia and stable vitiligo lesions in order to identify biomarkers that can accurately assess the risk of cancerous lesions of such lesions. Methods Taq Man low density miRNA microarray was used to analyze the molecular biology of stable leukoplakia and malignant leukoplakia. Results Compared with stable leukoplakia, the expression of miRNAs in cancerous leukoplakia significantly changed (P <0.05), and the expression of 13 miRNAs was down-regulated (miR-99a-5p, miR-99b-5p, miR -144 miR-100-5p miR-125-5p miR-181b miR-181a miR-521-3p miR-339-5p miR-15a-5p miR-150-5p let-7a -5p, miR-9), 12 miRNAs were upregulated (miR-10b-5p, miR-708, miR- 30e-3p, miR- 30a-3p, miR- 21, miR- 335-5p, miR- 144 , MiR-25-3p, miR-19a-3p, miR-660-5p, miR-140-5p, miR-590-5p). Conclusion The detection of miRNA biomarkers is of great significance for the diagnosis of stable leukoplakia and malignant leukoplakia.