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【目的】嫁接是繁殖薄壳山核桃的重要手段,拟从蛋白质水平揭示薄壳山核桃嫁接愈合的机理。【方法】利用双向电泳技术结合MALDI-TOF/TOF-MS,研究了薄壳山核桃嫁接愈合部位4个发育时期(嫁接后第1、6、10和25天)的差异蛋白。【结果】共成功鉴定48个差异蛋白,这些差异蛋白按功能可分为7类,包括能量代谢、抗性及防御、细胞生长、次生代谢、蛋白质合成、氨基酸代谢以及未知功能蛋白。【结论】果糖二磷酸醛缩酶、磷酸甘油酸激酶、丙酮酸脱羧酶、三磷酸腺苷酶能够为嫁接愈合提供所需的能量;抗坏血酸过氧化物酶、过氧化物还原酶能够有效清除嫁接愈合过程中积累过多的活性氧;可溶性无机焦磷酸酶可以促进愈伤组织的增殖;类胱天冬蛋白酶、α微管蛋白可能参与管状分子的分化。
【Objective】 Grafting is an important method to reproduce thin-walnut. It is proposed that the mechanism of grafting and healing of thin-walnut is revealed from the protein level. 【Method】 Two-dimensional electrophoresis combined with MALDI-TOF / TOF-MS was used to study the differentially expressed proteins at four developmental stages (1, 6, 10 and 25 days after grafting). 【Result】 A total of 48 differentially expressed proteins were successfully identified. These differentially expressed proteins were divided into 7 groups according to their functions, including energy metabolism, resistance and defense, cell growth, secondary metabolism, protein synthesis, amino acid metabolism and unknown function proteins. 【Conclusion】 Fructose bisphosphate aldolase, phosphoglycerate kinase, pyruvate decarboxylase and adenosine triphosphate enzyme can provide the necessary energy for graft healing; ascorbate peroxidase and peroxidase reductase can effectively remove the graft during healing process Accumulate too much reactive oxygen species; soluble inorganic pyrophosphatase can promote the proliferation of callus; caspases, α-tubulin may be involved in the differentiation of tubular molecules.