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在相同的激素浓度下,中国黄连叶片愈伤组织在B_5培养基上生长较好,且小檗碱含量明显增加。~60Coγ-射线的辐照剂量在5~40Gy范围内都能提高愈伤组织的小檗碱含量,其中以20Gy的辐照剂量效果为最好。
At the same hormone concentration, callus of Chinese Rhizoma coptidis leaves grew well on B5 medium, and the content of berberine increased significantly. The irradiation dose of ~60Co γ-rays can increase the berberine content of callus in the range of 5-40 Gy, and the irradiation dose of 20 Gy is the best.