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:山西省卫生防疫站脊髓灰质炎 (脊灰 )实验室从 1998年 7月正式使用L2 0B细胞 ,并同时使用RD和Hep - 2细胞进行急性弛缓性麻痹 (AFP)病例病原学监测 ,为了解同时使用 3种细胞对AFP病例病原学监测结果的影响 ,将 3 0 8例AFP病例粪便标本在 3种细胞上的分离状况进行比较分析。同时使用 3种细胞共分离到脊灰病毒 (PV)2 0份 ,其中RD细胞分离到 14份 ,L2 0B细胞 16份 ,Hep - 2细胞 8份 ,总分离率为 6 5 %。分离到非脊灰肠道病毒(NPEV) 65株 ,其中RD细胞 3 0株 ,Hep - 2细胞 4 8株 ,分离率为 2 1 1%。结果显示 ,同时使用 3种细胞对提高PV检测灵敏度和NPEV分离率 ,保证脊灰实验室监测质量有重要意义。
: Polio (polio) laboratory of Shanxi Provincial Health and Epidemic Prevention Station formally used L2 0B cells from July 1998 and concurrently used RD and Hep - 2 cells for etiological monitoring of acute flaccid paralysis (AFP) cases. To understand At the same time using three kinds of cells on the AFP case etiology monitoring results, the 388 cases of AFP stool specimens on the separation of three kinds of cells were analyzed. Poliovirus (PV) 20 were isolated from 3 kinds of cells. Among them, 14 were isolated from RD cells, 16 from L2 0B cells and 8 from Hep - 2 cells with a total isolation rate of 65%. Sixty - five isolates of non - polio enterovirus (NPEV) were isolated, of which 30 were RD cells and 48 were Hep - 2 cells with the isolation rate of 21.1%. The results showed that the simultaneous use of three kinds of cells to improve the PV detection sensitivity and NPEV isolation rate, to ensure the quality of polio laboratory monitoring is of great significance.