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目的观察鼠疫噬菌体对感染鼠疫小鼠的治疗作用,以寻找治疗鼠疫感染的新方法。方法小鼠经皮下感染141株鼠疫菌建立鼠疫感染模型后,用效价为10-11的鼠疫噬菌体对短期治疗组在感染后3、6、12和24 h给予100μl/次的注射治疗,对长期治疗组在感染后1～7 d连续注射鼠疫噬菌体,100μl/次,1次/d。同时,设阴性对照组(不感染,只治疗,观察噬菌体对小鼠的毒性)和阳性对照组(仅感染,不治疗)。结果阴性对照组小鼠治疗14 d后全部存活,处死后剖检未检出鼠疫菌。各治疗组小鼠在治疗第5天存活率为100%,与阳性对照组相比,差异有统计学意义(P<0.05);长期腹腔治疗组效果明显,鼠疫噬菌体在小鼠体内存活时间长,效价不降低。结论鼠疫噬菌体对感染小鼠急性感染期的治疗有一定效果,长期腹腔注射治疗效果较好,为其进一步应用于抗感染治疗提供了实验依据。
Objective To observe the therapeutic effect of plague bacteriophages on plague-infected mice in search of a new method to treat plague infection. Methods The mouse model of plague infection was established after 141 strains of P. infestans were subcutaneously infected. The mice in the short-term treatment group were immunized with 100 μl / injection at 3, 6, 12 and 24 hours after infected with the plague phage titer of 10-11. Long-term treatment group 1 to 7 days after infection, continuous injection of plague phage, 100μl / time, 1 time / d. At the same time, set negative control group (no infection, only treatment, observe phage toxicity in mice) and positive control group (infection only, no treatment). Results The mice in the negative control group all survived 14 days after treatment, and no Pseudomonas aeruginosa was detected by necropsy. Compared with the positive control group, the survival rate of the mice in each treatment group was 100% on the fifth day of treatment, the difference was statistically significant (P <0.05); long-term peritoneal treatment group effect was obvious, the plague phage in mice survived for a long time , Potency does not reduce. Conclusions The phage of the plague have some effects on the acute infection of infected mice. The long-term intraperitoneal injection of the bacteriophage has a good effect, which provides experimental evidence for its further application in anti-infective therapy.