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作者用自制的[~(125)I]标记激素,参考国外经验,建立了人血清胰岛素抗体、猪胰岛素原抗体和胰多肽抗体等三种放射免疫检测法,并对方法的主要实验条件进行了优选,对方法的质量控制参数作了验证。结果发现:在使用国产胰岛素的糖尿病人血清中,三种抗体的检出率分别为90.8％、48.3％和36.5％;抗体特异性结合值分别为21.2±17.4％、41.8±27.4％和25.6±28.4;而正常人和糖尿病人未用胰岛素者全部为阴性,显示国产胰岛素具有明显的免疫原性。
The author used homemade [~ (125) I] labeled hormones and made reference to foreign experience to establish three kinds of radioimmunoassay methods, including human serum insulin antibody, porcine proinsulin antibody and pancreatic polypeptide antibody. The main experimental conditions of the method Preferably, the method quality control parameters are validated. The results showed that the detection rates of the three antibodies were 90.8%, 48.3% and 36.5% respectively in the sera of diabetic patients using domestic insulin. The antibody specific binding values were 21.2 ± 17.4%, 41.8 ± 27.4% and 25.6 ± 28.4; while normal and diabetic patients without insulin were all negative, indicating that domestic insulin has obvious immunogenicity.