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目的分析TTV在新疆蒙古族、汉族肝炎患者的部分基因序列,为新疆各民族中TTV感染的流行病学调查提供可靠的依据。方法根据AB008394全基因序列设计引物,建立巢式聚合酶链反应(Nested-PCR),检测新疆地区各民族肝炎患者262例血清中的TTV DNA,对其中的11例TTV阳性者进行了克隆测序。结果本组11例部分基本序列与AB008394相比较,其核苷酸序列的同源性10例均在90％以上,仅蒙古族为87.1％。结论本研究证实新疆地区的蒙古族中存在TTV感染。
Objective To analyze the gene sequences of TTV in Mongolian and Han patients with hepatitis in Xinjiang and provide a reliable basis for the epidemiological investigation of TTV infection among ethnic groups in Xinjiang. Methods According to the sequence of AB008394, Nested-PCR was used to detect the TTV DNA in 262 serum samples from all ethnic groups in Hepatitis of Xinjiang. TTV positive samples were cloned and sequenced. Results The basic sequences of 11 cases of this group were compared with AB008394. The nucleotide sequence homology of all the 10 sequences was over 90%, while that of Mongolian was 87.1%. Conclusion This study confirmed the presence of TTV infection among Mongolians in Xinjiang.