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本研究选用昆明种小鼠,在妊娠6～14天,每天腹腔给予0、0.2、1.0和5.0mg/kg的V_2O_5,末次给药后15～18小时,分析母体及胚胎细胞的微核和SCE频率。结果:V_2O_5对SCE频率均无显著影响(P>0.05);但诱导了胎肝及母鼠脾脏、骨髓PEC的微核率的显著增加(P<0.05)。这表明,V_2O_5不仅对成体确具诱变性,而且还能透过胎盘,诱发胚胎细胞的染色体损伤。MNT结果还揭示:胎肝和孕鼠脾脏对化学物质的微核诱导作用较骨髓敏感;当MNT结果在界值附近时,最好采用AO染色法。
In this study, Kunming mice were used to inoculate 0, 0.2, 1.0 and 5.0 mg / kg of V 2 O 5 intraperitoneally daily from 6 to 14 days of gestation and from 15 to 18 hours after the last administration to analyze the micronuclei and SCE frequency. Results: V 2 O 5 had no significant effect on the frequency of SCE (P> 0.05). However, the micronuclei of PEC in fetal liver and maternal spleen and bone marrow were significantly increased (P <0.05). This indicates that V2O5 is not only mutagenic to the adult, but also penetrates the placenta to induce chromosome damage in embryonic cells. The results of MNT also revealed that micronuclei inducement of chemical substances in fetal liver and spleen of pregnant rats was more sensitive than that of bone marrow. AO staining was the best method when MNT was near the cutoff value.