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0 引言 应用免疫荧光组织化学方法结合激光扫描共聚焦显微镜 ( LSCM) ,观察了人乳头瘤病毒 HPV1 6型 ( Human pa-pillomavirus type1 6) E6,E7基因区表达的 E6,E7蛋白产物在宫颈癌细胞系 Si Ha中的表达情况 .1 材料和方法1 .1 细胞培养 含 HPV基因组织的宫颈癌细胞系 Si H
0 Introduction Immunofluorescence histochemistry and laser scanning confocal microscopy (LSCM) were used to observe the expression of E6 and E7 protein in E6 and E7 gene regions of human papillomavirus type 1 (6) Cell line Si Ha expression.1 Materials and methods 1.1 cell culture with cervical cancer cell lines containing HPV tissue Si H