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目的 :观察人类内源性逆转录病毒长末端重复序列 (humanretroviruslongterminalrepeat,HRTVLTR)在转移和非转移性肺癌中的表达 ,探讨HRTVLTR激活与肺癌转移的关系。方法 :采用PCR扩增 17例肺癌病人原发灶、邻近淋巴结及癌旁正常组织基因组中的内源性HRTVLTR ,证明内源性HRTVLTR的存在 ;以RNA斑点杂交检测 17例肺癌病人原发灶、邻近淋巴结及癌旁正常组织内源性HRTVLTR的表达 ,比较其在转移和非转移性肺癌中的表达差异。结果 :PCR结果显示 ,在转移能力不同的人肺腺癌细胞系 (AGZY 83a和Anip 973)及肺癌病人原发灶、邻近淋巴结及癌旁正常组织均获得了HRTVLTR扩增产物。 17例肺癌标本RNA斑点杂交分析发现 ,8例转移性非小细胞肺癌病人中有 5例原发灶及其转移淋巴结表现为HRTVLTR的高表达 ,4例小细胞肺癌无HRTVLTR的表达。结论 :人类基因中普遍存在内源性HRTVLTR ;HRTVLTR的高表达可能与非小细胞肺癌的转移有关
Objective: To observe the expression of human endogenous retrovirus long terminal repeat (HRTVLTR) in metastatic and non-metastatic lung cancer, and to explore the relationship between HTV TLR activation and lung cancer metastasis. METHODS: Endogenous HTVTVLTR was amplified from the primary tumor, adjacent lymph nodes, and normal tissue adjacent to tumor in 17 patients with lung cancer by PCR, demonstrating the presence of endogenous HTVTVLTR. The primary tumors of 17 lung cancer patients were detected by RNA dot blot hybridization. The expression of endogenous HTVTVLTR in adjacent lymph nodes and adjacent normal tissues was compared with that in metastatic and non-metastatic lung cancers. RESULTS: PCR results showed that HRVTVL amplified products were obtained in human lung adenocarcinoma cell lines (AGZY 83a and Anip 973) with different metastasis capacity, primary tumors, adjacent lymph nodes and normal tissues adjacent to lung cancer. The RNA dot blot analysis of 17 lung cancer specimens found that 5 out of 8 patients with metastatic NSCLC showed high expression of HRTVLTR in the primary tumor and its metastatic lymph nodes, and 4 cases of small cell lung cancer had no expression of HRVTVLTR. Conclusion: Endogenous HTVTVLTR is prevalent in human genes; high expression of HTVTVLTR may be related to the metastasis of non-small cell lung cancer.