目的为减少单采血小板细菌污染,对供应临床的部分单采血小板开展细菌检测,保障临床用血安全。方法使用法国生物梅里埃Bac T/ALERT 3D 240全自动微生物检测系统、需氧培养瓶(BPA)和厌氧培养瓶(BPN)对单采血小板进行细菌检测,细菌培养阳性进行转种,转种阳性进行细菌菌种鉴定;培养7d阴性为单采血小板无菌生长。结果 6年间共对14 923袋单采血小板进行细菌检测,其中发现有菌生长17例,阳性率为0.11%。专性厌氧菌9例(53%),需氧菌8例(47%)。结论尽管应用先进的血液培养技术,遵照新的质量标准,然而,细菌污染及脓毒性输血反应仍然是血小板输血所面临的重要风险。因此,采供血机构需要不断改进细菌污染检测技术,完善质量控制程序,并逐步建立PLT输血安全监测体系。 Objective To reduce the contamination of apheresis bacteria, the clinical supply of partial apheresis plate to carry out bacterial testing to ensure the safety of clinical blood use. Methods Bacteria were detected by Bac T / ALERT 3D 240 automatic biomonitoring system, aerobic culture flask (BPA) and anaerobic flask (BPN). The bacteria were cultured and transplanted Positive bacterial species identification; culture 7d negative platelet aseptic growth. Results A total of 14 923 bags of apheresis platelets were tested for bacteria during the past six years. Among them, 17 cases were found to have bacterial growth with a positive rate of 0.11%. There were 9 obligate anaerobic bacteria (53%) and 8 aerobic bacteria (47%). Conclusion Despite the application of advanced blood culture techniques and adherence to new quality standards, bacterial contamination and septic transfusion reactions remain an important risk for platelet transfusions. Therefore, blood collection and supply agencies need to continuously improve bacterial contamination detection technology, improve quality control procedures, and gradually establish PLT blood transfusion safety monitoring system.