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主要建立了HPLC法测定大豆提取物中大豆异黄酮含量的方法,通过实验确定了HPLC法测定大豆提取物中大豆异黄酮的最佳条件是:采用Waters Symmetry 5μm C18,250 mm×4.6 mm色谱柱;流速:1.0 mL/min;波长260 nm;柱温:35℃;流动相A为含0.1%(v/v)乙酸的乙腈溶液,流动相B为含0.1%(v/v)乙酸的超纯水溶液,流动相梯度:18%～35%的乙腈溶液。该方法灵敏度高、重现性好,回收率达98%,变异系数小于3%,为研究大豆异黄酮其它产品打下了良好的基础,操作方法简单,易于掌握。
The main method for the determination of soybean isoflavone content in soybean extract by HPLC was established. The optimum conditions for the determination of soybean isoflavone in soybean extract by HPLC were as follows: using a Waters Symmetry 5μm C18, 250 mm × 4.6 mm column ; Flow rate: 1.0 mL / min; wavelength 260 nm; column temperature: 35 ° C .; mobile phase A was acetonitrile containing 0.1% (v / v) acetic acid and mobile phase B Pure water solution, mobile phase gradient: 18% ~ 35% acetonitrile solution. The method has high sensitivity, good reproducibility, 98% recovery rate and a coefficient of variation of less than 3%, laying a good foundation for the research of other products of soybean isoflavones. The method is simple and easy to master.