论文部分内容阅读
目的了解胃癌组织中环氧酶-2(COX-2)和白细胞介素-17(IL-17)的表达,COX-2的表达与胃癌临床病理特征间的关系及两者的相关性。方法收集60例胃癌患者和20例健康对照者的组织标本。胃癌患者包括男44例,女16例,平均年龄(61.8±10.9)岁。根据TNM分期将胃癌分为TNMⅠ期(7例)、Ⅱ期(8例)、Ⅲ期(32例)和Ⅳ期(13例);按组织分化程度分为低分化组(34例)和中-高分化组(26例);按发病部位分为贲门癌(7例)和非贲门癌(53例);按有无淋巴结转移分为有转移(43例)和无转移(17例)。采用免疫组织化学染色测定胃癌组织中COX-2和IL-17的表达。结果胃癌组织中COX-2和IL-17的表达明显高于对照组,胃癌组织中COX-2的表达水平与胃癌的临床分期、有无淋巴结转移、肿瘤大小及分化程度有关(P<0.01),与患者的年龄、性别、肿瘤部位无关(P>0.05)。胃癌组织中COX-2的表达水平与IL-17的表达水平呈正相关(P<0.001)。结论 COX-2和IL-17在胃癌的发生和发展中起促进作用,COX-2和IL-17可能互为正向调节因素。
Objective To investigate the expression of cyclooxygenase-2 (COX-2) and interleukin-17 (IL-17) in gastric cancer and the relationship between the expression of COX-2 and clinicopathological features of gastric cancer. Methods Tissue samples of 60 patients with gastric cancer and 20 healthy controls were collected. Gastric cancer patients, including 44 males and 16 females, mean age (61.8 ± 10.9) years. According to TNM stage, gastric cancer was divided into TNM stage Ⅰ (7 cases), stage Ⅱ (8 cases), stage Ⅲ (32 cases) and stage Ⅳ (13 cases). According to the degree of differentiation, gastric cancer was divided into low differentiation group - well-differentiated group (26 cases); divided into cardia cancer (7 cases) and non-cardiac cancer (53 cases) according to the site of disease; divided into metastasis (43 cases) and no metastasis (17 cases) by lymph node metastasis. The expression of COX-2 and IL-17 in gastric cancer tissue was detected by immunohistochemistry. Results The expression of COX-2 and IL-17 in gastric cancer tissues was significantly higher than that in the control group. The expression of COX-2 in gastric cancer tissues was correlated with the clinical stage, lymph node metastasis, tumor size and differentiation (P <0.01) , Not related to the patient’s age, sex, tumor site (P> 0.05). The expression of COX-2 in gastric cancer tissues was positively correlated with the expression of IL-17 (P <0.001). Conclusions COX-2 and IL-17 play a role in the carcinogenesis and progression of gastric cancer. COX-2 and IL-17 may play a positive role in the regulation of gastric cancer.