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利用人工智能模拟霜箱,设8个温度(18、–1、–2、–3、–4、–5、–6、–7℃),对抗寒性不同的两个仁用杏(Prunus armeniaca Linn.)品种雌蕊的过氧化氢(H2O2)含量和抗坏血酸—谷胱甘肽(AsA-GSH)循环进行了研究。结果表明,低温胁迫增加了仁用杏雌蕊H2O2含量,–7℃时抗晚霜品种‘围选1号’和晚霜敏感品种‘龙王帽’分别为对照(18℃)的5.49倍和7.22倍。低温期间,‘围选1号’雌蕊AsA等酶含量及APX等酶活性均高于‘龙王帽’,–7℃时,‘围选1号’雌蕊AsA、MDHA、DHA、GSH和GSSG含量比对照分别下降了66%、69%、48%、52%、53%,‘龙王帽’下降了78%、79%、59%、66%、65%,–7℃时,‘围选1号’雌蕊APX、MDHAR、DHAR、GPX、GR活性、AsA/DHA、GSH/GSSG分别是对照的2.87倍、1.07倍、1.42倍、1.60倍、1.20倍、65%和1.03倍,‘龙王帽’是对照2.41倍、96%、1.13倍、1.08倍、98%、54%、97%。结果表明‘围选1号’雌蕊在低温期间有较高的AsA-GSH循环效率,可有效抑制H2O2的积累,这可能是其抗晚霜能力较强的重要原因之一。
Using artificial intelligence to simulate the frost box, eight different temperatures (18, -1, -2, -3, -4, -5, -6 and -7 ℃) Hydrogen peroxide (H2O2) content and ascorbate-glutathione (AsA-GSH) cycling in the pistil of Linn. The results showed that low temperature stress increased the content of H2O2 in pistil of apricot apricot, and was 5.49-fold and 7.22-fold higher than the control (18 ℃) at -7 ℃, respectively. At low temperature, the contents of AsA and other enzymes and the activities of APX and other enzymes in the ’pomegranate No.1’ pistil were higher than those in the ’Dragon King’ hatch. At -7 ℃, the content of AsA, MDHA, DHA, GSH and GSSG The control dropped by 66%, 69%, 48%, 52%, 53%, ’Dragon King’ dropped 78%, 79%, 59%, 66%, 65% ’Pokey APX, MDHAR, DHAR, GPX, GR activity, AsA / DHA, GSH / GSSG were 2.87 times, 1.07 times, 1.42 times, 1.60 times, 1.20 times, 65% and 1.03 times of the control, Controls were 2.41 times, 96%, 1.13 times, 1.08 times, 98%, 54%, 97%. The results showed that the ploidy of ’Guanyuan 1’ had higher AsA-GSH cycle efficiency at low temperature, which could effectively inhibit the accumulation of H2O2, which may be one of the important reasons for its strong resistance to night frost.