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目的:观察补青颗粒对大鼠半乳糖性白内障的防治作用。方法:用SD大鼠复制半乳糖白内障模型。实验分为6组:空白对照组无特殊处理;模型对照组每日ip注射50%D-半乳糖溶液(10 g.kg-1),连续15 d,制成白内障动物模型;阳性对照组同模型组处理的同时每天给予障眼明片混悬液0.02 g.kg-1ig至实验结束;补青颗粒高、中、低剂量组处理同模型组且每天分别给予0.8,0.4,0.2 g.kg-1的剂量ig至实验结束。分别记录实验前及造模起第3,7,14天晶状体的混浊程度,实验结束时检测各组晶体超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果:对照组晶状体始终透明,模型组第3天晶体开始发生混浊,补青颗粒3个剂量组晶体混浊的速度及程度在3,7,14天均明显低于模型组(P<0.05),SOD活性显著高于模型组(P<0.05),MDA含量显著低于模型组(P<0.05)。结论:补青颗粒具有较好的防治大鼠半乳糖性白内障的作用。
Objective: To observe the prevention and treatment of Buqing granule on rat galactose cataract. Methods: Galactose cataract model was duplicated with SD rats. The experiment was divided into 6 groups: the blank control group without special treatment; the model control group were injected with 50% D-galactose solution (10 g.kg-1) daily for 15 days to make a cataract animal model; At the same time, the rats in the model group were given 0.02g.kg-1ig of barrier ophthalmic solution every day until the end of the experiment. The rats in high, medium and low dose group were treated with the same model group and given 0.8, 0.4 and 0.2 g.kg -1 dose ig until the end of the experiment. The degree of opacity of the lens was recorded before the experiment and on the 3rd, 7th and 14th day after the model was established. At the end of the experiment, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured. Results: The lens of the control group was always transparent. The opacity began to occur on the third day in the model group. The speed and degree of crystal opacity in the three dosage groups of the QingQing granules group were significantly lower than those of the model group at 3, 7 and 14 days (P <0.05) SOD activity was significantly higher than the model group (P <0.05), MDA content was significantly lower than the model group (P <0.05). CONCLUSION: Buqing granule has good effect in preventing and treating galactose cataract in rats.