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目的动态观察多房棘球绦虫重组BCG-EmⅡ/3疫苗免疫小鼠后脾细胞亚群的变化。方法将疫苗采用鼻腔内接种免疫Balb/c鼠,在免疫后0、2、4、6、8、10、12、14、16周和18周各剖杀4只小鼠取脾,分离脾细胞,流式细胞仪检测脾CD4+和CD8+T淋巴细胞亚群的百分比,同时设有PBS对照。结果疫苗接种组的脾CD4+和CD8+T细胞亚群分别在免疫后2～10周和2～18周升高,分别在免疫后4周和10周达最高水平。结论多房棘球绦虫重组BCG-EmⅡ/3疫苗可诱导小鼠脾CD4+和CD8+T细胞亚群的增殖。
Objective To observe the changes of splenocyte subsets in mice immunized with recombinant BCG-EmⅡ / 3 vaccine of Echinococcus multilocularis. Methods Balb / c mice were immunized intranasally with the vaccine and four mice were sacrificed at 0, 2, 4, 6, 8, 10, 12, 14, 16 and 18 weeks after immunization. The percentages of splenic CD4 + and CD8 + T lymphocyte subsets were detected by flow cytometry with PBS control. Results The levels of splenic CD4 + and CD8 + T cells in the vaccinated group increased at 2 ~ 10 weeks and 2 ~ 18 weeks after immunization, reaching the highest level at 4 weeks and 10 weeks after immunization. Conclusion The recombinant BCG-EmⅡ / 3 vaccine of Echinococcus multilocularis can induce the proliferation of splenic CD4 + and CD8 + T cell subsets in mice.