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目的研究放射线对鼻咽癌CNE细胞细胞周期阻滞、凋亡和抑癌基因p57kip2蛋白表达的影响。方法运用流式细胞术检测放射线诱导的细胞周期阻滞、凋亡;运用免疫组织化学法检测抑癌基因p57kip2蛋白的表达。结果CNE细胞经照射后,G1期无明显阻滞,S期出现短暂堆积,G2/M期阻滞程度具剂量和时间依赖性,G2/M期阻滞在12h与24h时与照射剂量呈正相关(P<0.01),随照射后时间延长而增强,峰值出现于12Gy24h;细胞凋亡发生率与照射剂量和照射后时间均呈正相关(P<0.01)。照射后p57kip2蛋白表达随照射剂量增加和照射后时间延长而上调(均P<0.01)。结论G2/M期阻滞、细胞凋亡率和p57kip2蛋白均能反映及预测鼻咽癌细胞的放射敏感性。kip2
Objective To investigate the effect of radiation on the cell cycle arrest, apoptosis and expression of p65kip2 protein in nasopharyngeal carcinoma CNE cells. Methods The cell cycle arrest and apoptosis induced by radiation were detected by flow cytometry. The expression of the tumor suppressor gene p57kip2 protein was detected by immunohistochemistry. Results After irradiation, CNE cells showed no obvious arrest in G1 phase, transient accumulation in S phase, and G2 / M arrest in a dose and time dependent manner. G2 / M arrest was positively correlated with irradiation dose at 12h and 24h (P <0.01). The peak appeared at 12Gy24h after irradiation. The incidence of apoptosis was positively correlated with dose and time (P <0.01). After irradiation, the expression of p57kip2 protein was up-regulated with the increase of irradiation dose and the prolongation of irradiation time (all P <0.01). Conclusion G2 / M phase arrest, apoptosis rate and p57kip2 protein both reflect and predict radiosensitivity of nasopharyngeal carcinoma cells. kip2