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目的评价滇结香花不同提取部位对四氯化碳诱导小鼠肝损伤的影响。方法取昆明种小鼠110只,随机分为空白对照组,模型组,联苯双酯组(阳性对照组,0.15 g·kg~(-1))以及滇结香花石油醚提取部位、30%乙醇提取部位、60%乙醇提取部位、90%乙醇提取部位的低、高剂量组(给药量分别为0.1,0.3 g·kg~(-1)),各剂量组连续给药7 d,腹腔注射0.2%四氯化碳花生油溶液(10 mL·kg~(-1))建立急性肝损伤小鼠模型,眼眶取血,测定血清中ALT、AST、TBIL、TBA的含量变化;小鼠肝脏处理,测定肝组织匀浆GSH、MDA、SOD含量,观察四氯化碳诱导急性肝损伤小鼠肝组织病理学变化。结果滇结香花不同提取部位不同剂量组中,除石油醚部位低剂量组、90%乙醇提取部位低剂量组外,其他剂量组均可显著降低血清中ALT、AST、TBIL水平(P<0.01);除石油醚低、高剂量组外,其他剂量组均可降低血清TBA水平(P<0.05,P<0.01)。测定肝组织匀浆中各项指标,石油醚高剂量组和30%及60%乙醇提取部位低、高剂量组中,MDA水平明显降低,GSH、SOD水平显著升高(P<0.05,P<0.01);小鼠肝组织病理学变化,除石油醚低剂量组、30%乙醇提取部位低剂量组及90%乙醇提取部位低、高剂量组外,其他剂量组损伤均有明显缓解。结论滇结香花30%乙醇提取部位高剂量组及60%乙醇提取部位低、高剂量组具有保护肝组织的作用,缓解四氯化碳造成的肝损伤,并且60%乙醇提取部位其作用效果明显优于阳性对照组。其机制可能是通过阻断脂质过氧化反应,清除体内过量的自由基,保护细胞的正常代谢,维持细胞的稳定性,该研究为一种新的用药资源提供了一定的理论依据。
Objective To evaluate the effects of different extracts from Fructus Juniperus on liver injury induced by carbon tetrachloride in mice. Methods A total of 110 Kunming mice were randomly divided into three groups: control group, model group, bifendate group (0.15 g · kg -1), and 30% Ethanol extract, 60% ethanol extract, 90% ethanol extract of the low and high dose groups (dose were 0.1,0.3 g · kg -1), the dose of 7 consecutive days of administration, abdominal cavity The model of acute liver injury was established by injecting 0.2% carbon tetrachloride peanut oil solution (10 mL · kg -1) into the orbital and the orbital blood was collected to determine the content of ALT, AST, TBIL and TBA in serum. The content of GSH, MDA and SOD in liver homogenate were measured. The pathological changes of liver in mice induced by carbon tetrachloride were observed. Results The levels of ALT, AST and TBIL in serum of different dosage groups were significantly lower than those of low dosage of petroleum ether fraction and low dosage of 90% ethanol extract (P <0.01) (P <0.05, P <0.01), except the petroleum ether low and high dose groups. The levels of MDA, GSH and SOD in the high-dose petroleum ether group and the low-dose and high-dose groups of 30% and 60% ethanol extract were significantly lower than those in the high-dose petroleum ether group (P < 0.01). The histopathological changes of liver in mice except the petroleum ether low dose group, 30% ethanol extract low dose group and 90% ethanol extract low and high dose group, the other dose group were significantly relieved. Conclusions The low dose and high dose of 30% ethanol extract of highland and low dose of 60% ethanol extract can protect hepatic tissue and relieve liver injury caused by carbon tetrachloride, and the effect of 60% ethanol extraction is obvious Better than the positive control group. The mechanism may be through blocking lipid peroxidation, scavenging excess free radicals in the body, protecting the normal metabolism of cells and maintaining cell stability. This study provides a theoretical basis for a new drug resource.