论文部分内容阅读
目的观察药理剂量糖皮质激素对大鼠生长板软骨细胞糖皮质激素受体的调节作用;探究糖皮质激素抑制大鼠骨骼纵向生长的机制。方法 3周龄清洁级纯系雄性 SD 大鼠,实验组12只,地塞米松(200μg/100g,10d)处理;对照组8只,注射等容积的生理盐水。测量大鼠胫骨长。胫骨冠状面切片。Mallory 三色法染色观测生长板组织学,测量生长板总厚度、增殖带、肥大带厚度。糖皮质激素受体免疫组化分析。结果 (1)实验组大鼠胫骨长、生长板总厚度、增殖带、肥大带厚度分别为28.9mm±1.2mm,4.01μm±0.28μm,1.98μm±0.13μm,1.67μm±0.1μm;均明显落后于对照组30.5mm±1.1mm,5.53μm±0.46μm,2.25μm±0.30μm,2.87μm±0.19μm,差异均有统计学意义(均 P<0.05)。(2)生长板静止带和肥大带都表达糖皮质激素受体;而增殖带未见糖皮质激素受体表达。(3)地塞米松处理使生长板静止带和肥大带软骨细胞糖皮质激素受体表达增强。结论糖皮质激素受体是骨骼纵向生长所必需的。药理剂量糖皮质激素通过上调静止带和肥大带软骨细胞糖皮质激素受体抑制骨骼纵向生长。
Objective To observe the regulatory effect of pharmacological glucocorticoid on glucocorticoid receptor of rat growth plate and to explore the mechanism of glucocorticoid inhibiting bone longitudinal growth in rats. Methods 3-week-old clean male SD rats were randomly divided into experimental group (n = 12) and dexamethasone (200μg / 100g, 10d). Measurement of rat tibia length. Tibial coronal section. Mallory trichrome staining of growth plate histology, total growth plate thickness, proliferation zone, hypertrophy zone thickness. Immunohistochemical analysis of glucocorticoid receptor. Results (1) The tibia length, total thickness of growth plate, proliferation zone and hypertrophy zone in experimental group were 28.9mm ± 1.2mm, 4.01μm ± 0.28μm, 1.98μm ± 0.13μm, 1.67μm ± 0.1μm respectively; Lagging behind the control group 30.5mm ± 1.1mm, 5.53μm ± 0.46μm, 2.25μm ± 0.30μm, 2.87μm ± 0.19μm, the differences were statistically significant (P <0.05). (2) Growth plate resting zone and hypertrophic zone express glucocorticoid receptor, while no proliferative zone express glucocorticoid receptor. (3) Dexamethasone treatment enhanced the expression of glucocorticoid receptor in resting zone and hypertrophic chondrocytes in growth plate. Conclusion Glucocorticoid receptors are required for the longitudinal growth of bone. Pharmacological doses of glucocorticoids inhibit skeletal longitudinal growth by up-regulating the resting zone and hypertrophic chondrocyte glucocorticoid receptor.