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[目的]对手足口病患者标本进行病毒核酸检测,探讨实时荧光定量RT-PCR方法在手足口病疫情监测中的意义。[方法]采用实时荧光定量RT-PCR方法对2010年3～8月连云港市采集的307份手足口病患者标本进行肠道病毒核酸检测,并对肠道病毒71型(EV71)、柯萨奇病毒A组16型(CVA16)进行分型。[结果]307份手足口病患者标本中有221份肠道病毒核酸阳性,阳性率71.99%,其中EV71型病毒核酸阳性标本123份,阳性率40.07%,CVA16型病毒核酸阳性标本66份,阳性率21.50%。[结论]实时荧光定量RT-PCR方法耗时短、特异性强、灵敏度高,可作为手足口病疫情监测的诊断方法。
[Objective] To detect virus nucleic acid in hand, foot and mouth disease patients and explore the significance of real-time fluorescence quantitative RT-PCR in the monitoring of HFMD. [Method] The real-time fluorescence quantitative RT-PCR method was used to detect the enterovirus nucleic acid of 307 hand-foot-mouth disease samples collected from Lianyungang in March to August in 2010, and the detection of enterovirus nucleic acid of enterovirus 71 (EV71) Virus A Group 16 (CVA16) was typed. [Results] 221 samples of hand, foot and mouth disease were positive for enterovirus nucleic acid, with a positive rate of 71.99%, of which 123 were positive for EV71 virus and 40.07% for positive samples, and 66 were positive for CVA16. Rate 21.50%. [Conclusion] The real-time fluorescent quantitative RT-PCR method is short, time-consuming, specific and sensitive and can be used as a diagnostic method for HFMD surveillance.