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来自巴西橡胶树同一基因型的不同松散型愈伤组织系可能会具有不同的胚胎发生及再生潜力,但从外观上却很难对其加以区分。为了深入揭示不同愈伤组织系之间所存在的差异,我们对5个具有不同胚胎发生及再生潜力的愈伤组织系进行了组织及分子学比较研究。试验中我们采用差异显示(DD-RT)技术对诱导期间差异表达的基因进行了分析。在胚性再生型愈伤组织系(ER)诱导期间共鉴定出28个差异表达的cDNA。此外还发现,ER系中胚胎发生结节(胚性结节)的形成要早于胚性非再生型愈伤组织系(ENR),而非胚性愈伤组织系(NE)中则根本不形成胚性结节。以上28个cDNAs中,有5个可用于诱导之前区分具有不同胚胎发生潜力的愈伤组织系,从而可以对橡胶树松散型愈伤组织的胚胎发生潜力作出提前判断。
Different loose callus lines from the same genotype of T. brasiliensis may have different potential for embryogenesis and regeneration, but they are difficult to distinguish from appearance. In order to reveal the differences between different callus lines, we conducted a comparative tissue and molecular study on five callus lines with different potential of embryogenesis and regeneration. In the experiment, we used differential display (DD-RT) to analyze differentially expressed genes during induction. A total of 28 differentially expressed cDNAs were identified during embryogenic regenerative callus induction (ER). In addition, it was also found that embryonic nodules (embryogenic nodules) were formed earlier in the ER line than in the embryogenic non regenerative callus (ENR) and not in the embryogenic callus (NE) Formation of embryonic nodules. Five of the above 28 cDNAs were used to induce previously differentiated callus lines with different embryogenic potential, allowing early judgment of the embryogenic potential of Loose Rubber Callus.