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自1890年 VonBehring 发现抗毒素以来,免疫学家们为获得单特异性抗体作过许多努力,均未成功。直至1975年,英国的Khler 和 Milstein 用小鼠骨髓瘤细胞与免疫小鼠的脾细胞(内含大量能分泌抗体的 B 细胞)杂交,成功地获得了抗羊红细胞的单克隆抗体,创立了淋巴细胞杂交瘤技术。该技术是利用亲代骨髓瘤细胞在体外能迅速生长繁殖,不分泌或缺乏 HGPRT
Since the discovery of anti-toxin by Von Behring in 1890, immunologists have made many efforts to obtain monospecific antibodies and have not succeeded. Until 1975, Khler and Milstein in the United Kingdom succeeded in obtaining monoclonal antibodies against sheep erythrocytes by hybridizing mouse myeloma cells with splenocytes of immunized mice (containing a large amount of antibody capable B cells), creating lymphoid Cell hybridoma technology. The technology is the use of parental myeloma cells in vitro rapid growth and reproduction, does not secrete or lack of HGPRT