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目的 研究Ag85B MPT6 4 (AM)融合基因疫苗对鼠结核分枝杆菌感染的保护效果。方法 C5 7BL/ 6小鼠 6 3只 ,随机分为磷酸盐缓冲液 (PBS)、空质粒、卡介苗 (BCG)、pcDNA/Ag85B、pcDNA/MPT6 4、pcDNA/Ag85B +pcDNA/MPT6 4、pcDNA/AM组 ,采用肌肉注射法免疫小鼠 ,0、3、6周各 1次 ,BCG组只在 0周予皮内注射卡介苗 1次。末次免疫后第 5周用H37Rv经尾静脉注射实施攻击 ,攻击后 6周处死部分小鼠 ,测血清总IgG ,特异性脾淋巴细胞增殖、IFN γ及IL 4分泌水平 ;观测脾、肺组织荷菌量和病理学检查以及剩余小鼠的存活时间。结果 AM基因疫苗组诱导的特异性的IgG、脾淋巴细胞增殖和IFN γ的分泌以及肺、脾组织荷菌量、病理学改变和小鼠存活时间明显优于其他DNA疫苗单独免疫组。结论 AMDNA疫苗在抗结核分枝杆菌感染过程中具有明显的免疫保护作用。
Objective To study the protective effect of Ag85B MPT6 4 (AM) fusion gene vaccine against Mycobacterium tuberculosis infection in mice. Methods Sixty-three C5 7BL / 6 mice were randomly divided into PBS, empty plasmid, BCG, pcDNA / Ag85B, pcDNA / MPT6 4, pcDNA / Ag85B + pcDNA / AM group, the mice were immunized by intramuscular injection, once every one, three, six and six weeks. The BCG group was intradermally injected with BCG only once a week. At the fifth week after the last immunization, H37Rv was injected through the tail vein for injection. Some mice were sacrificed 6 weeks after the challenge. Total serum IgG, specific splenic lymphocyte proliferation, IFNγ and IL 4 secretion were measured. Spleen and lung tissue load Bacterial volume and pathological examination as well as survival time of remaining mice. Results The specific IgG, splenic lymphocyte proliferation and IFNγ secretion induced by AM gene vaccine group and the bacterial load, pathological changes and survival time of mice in lung and spleen tissues were significantly better than those of other DNA vaccine immunized groups. Conclusion AM DNA vaccine has obvious immunoprotective effect in the course of anti-TB Mycobacterium infection.