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目的:研究蝉蜕、僵蚕对大鼠系膜增生性肾小球肾炎(MsPGN)的治疗作用。方法:采用改良慢性血清病法制备的MsPGN模型,随机分为模型对照组、蝉蜕高剂量组、蝉蜕低剂量组、僵蚕高剂量组、僵蚕低剂量组,另设正常对照组。分别在用药5周、8周后检测大鼠24小时尿蛋白;8周后处死大鼠,行血液生化指标检测和肾组织HE染色观察,免疫组化法检测TGF-β1的表达水平。结果:与模型对照组比较,用药5周后蝉蜕、僵蚕高剂量组显著降低大鼠24小时蛋白尿(P<0.01);8周后各治疗组大鼠肾组织TGF-β1表达量,24小时蛋白尿和血清胆固醇均显著下降,蝉蜕高、低剂量组血清白蛋白均有所升高,差异具有显著性意义(P<0.01)。肾组织形态学观察显示,蝉蜕、僵蚕高剂量组个别区域肾小球系膜细胞轻度增生,系膜区轻度增宽,管腔无挤压现象,较模型组明显改善。结论:蝉蜕、僵蚕均能有效降低MsPGN大鼠24小时尿蛋白,改善脂质代谢,其作用机制可能与抑制TGF-β1的过度表达有关。
Objective: To study the therapeutic effect of cicada sloughing and silkworm silkworm on mesangial proliferative glomerulonephritis (MsPGN) in rats. Methods: The MsPGN model prepared by modified chronic serodiagnosis was randomly divided into model control group, high dose group of cicadas, low dose group of cicadas, low dose of silkworm concanavalin group and low dose of silkworm. The rats were sacrificed after 8 weeks and 8 weeks respectively. After 8 weeks, the rats were sacrificed and the blood biochemical indexes and renal tissue HE staining were observed. The expression of TGF-β1 was detected by immunohistochemistry. Results: Compared with the model control group, the high-dose silkworm concanavalin group and the high-dose silkworm concanavalin group significantly reduced the 24-hour proteinuria (P <0.01). After 8 weeks, the expression of TGF- Hour proteinuria and serum cholesterol were significantly decreased, cicada sloughing, low-dose serum albumin were increased, the difference was significant (P <0.01). Morphological observation of kidney showed that glomerulus mesangial cells were slightly hyperplastic in some areas of the high-dose group and those in the high-dose group of cicadas and silkworms. The mesangial area was mildly broadened and no lumen was squeezed, which was significantly improved compared with the model group. CONCLUSION: Cicadae and silkworm can effectively reduce 24-hour urinary protein and improve lipid metabolism in MsPGN rats. The mechanism may be related to the inhibition of TGF-β1 overexpression.