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目的:评价微血管密度(MVD)的临床意义,比较分析CD105、VEGF、p53蛋白之间的相关性,探讨三者在喉癌肿瘤血管生成中的相互关系及临床病理意义。方法:采用免疫组织化学技术检测以CD105标记的MVD(CD105-MVD)、VEGF、p53蛋白在喉癌组织中的表达。结果:40例喉癌组织CD105-MVD为14.90±7.40,明显高于对照组织(P<0.05);Ⅲ、Ⅳ期喉癌组织的MVD高于Ⅰ、Ⅱ期,颈淋巴结转移组高于无淋巴结转移组,其差异有统计学意义;VEGF阳性率77.5%。p53蛋白阳性率67.5%。VEGF表达与肿瘤的TNM分期及病理分化有显著相关性(P<0.05)。p53蛋白表达与淋巴结转移呈显著相关(P<0.05)。CD105-MVD与VEGF、p53蛋白之间呈明显正相关(r=0.641,P<0.01;r=0.534,P<0.01)。结论:CD105与肿瘤活性血管生成有密切联系,是肿瘤新生血管的标志;喉癌组织CD105标记MVD的测定可作为预测喉癌患者复发转移及评估预后的重要独立指标;喉癌中VEGF的表达、p53蛋白表达与CD105-MVD呈正相关,三者联合可作为判断喉癌侵袭转移及预后的重要指标。
Objective: To evaluate the clinical significance of microvessel density (MVD), and to analyze the correlation between CD105, VEGF and p53 protein and to explore the relationship between them and their clinicopathological significance in angiogenesis of laryngeal carcinoma. Methods: Immunohistochemical technique was used to detect the expression of MVD (CD105-MVD), VEGF and p53 in the laryngeal squamous cell carcinoma. Results: The CD105-MVD in 40 laryngeal squamous cell carcinoma tissues was significantly higher than that in the control tissues (14.90 ± 7.40, P <0.05). The MVD in stages Ⅲ and Ⅳ laryngeal squamous cell carcinoma was higher than that in stage Ⅰ and Ⅱ, Metastasis group, the difference was statistically significant; VEGF positive rate of 77.5%. The positive rate of p53 protein was 67.5%. There was a significant correlation between VEGF expression and tumor TNM stage and pathological differentiation (P <0.05). There was a significant correlation between p53 protein expression and lymph node metastasis (P <0.05). There was a significant positive correlation between CD105-MVD and VEGF and p53 (r = 0.641, P <0.01; r = 0.534, P <0.01). Conclusions: CD105 is closely related to tumor angiogenesis and is a marker of neovascularization. CD105-labeled MVD in laryngeal carcinoma can be used as an important independent prognostic indicator in laryngeal cancer patients. VEGF expression, p53 protein expression and CD105-MVD was positively correlated, the combination of the three can be used as an important indicator of invasion and metastasis and prognosis of laryngeal cancer.