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目的探讨紫杉醇对人前列腺癌细胞PC-3增殖的体内抑制作用。方法建立体内绿色荧光蛋白(GFP)标记的人雄激素非依赖性前列腺癌细胞PC-3裸鼠原位移植瘤模型,观察紫杉醇对裸鼠前列腺癌原位移植瘤的体积、重量的影响。结果裸鼠模型体内实验显示,与对照组(100μL生理盐水)相比,紫杉醇处理组(0.5 mg/kg)在给药第18天后能显著抑制前列腺肿瘤的体积(P<0.05);紫杉醇处理组在抑制前列腺肿瘤重量方面与对照组相比亦有明显抑制作用(P<0.05)。与对照组相比G31P处理组VEGF(P<0.05)的表达差异具有统计学意义(免疫组化法)。结论紫杉醇在体内实验中能明显抑制人雄激素非依赖性前列腺癌细胞系PC-3的增殖。
Objective To investigate the inhibitory effect of paclitaxel on the proliferation of human prostate cancer cell line PC-3 in vitro. Methods To establish a green fluorescent protein (GFP) labeled human androgen-independent prostate cancer cell line PC-3 xenotransplanted in nude mice model and observe the effect of paclitaxel on the volume and weight of orthotopic transplanted tumor in nude mice. Results In vivo experiments in nude mice model showed that the paclitaxel treatment group (0.5 mg / kg) significantly reduced the volume of prostate tumors (P <0.05) on day 18 after administration compared with the control group (100 μL normal saline) In inhibiting prostate tumor weight compared with the control group also had significant inhibitory effect (P <0.05). Compared with the control group, the expression of VEGF (P <0.05) in G31P group was statistically significant (immunohistochemistry). Conclusion Paclitaxel can significantly inhibit the proliferation of human androgen-independent prostate cancer cell line PC-3 in vivo.