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目的探讨沉默 Caveolin-1(Cav-1)基因对人膀胱癌细胞株5637增殖和凋亡的影响。方法通过siRNA转染人膀胱癌细胞株5637(沉默组),以转染 FAM-siRNA为阴性对照组,未转染细胞为正常对照组。Re-al-time PCR法检测Cav-1基因沉默的效率,CCK-8法检测各组细胞的增殖情况,流式细胞术检测各种细胞的凋亡情况。结果 siRNA转染可显著降低人膀胱癌细胞株5637中 Cav-1 mRNA 的表达水平。沉默组细胞的增强能力明显减低,而细胞凋亡率明显升高(均P<0.05)。结论沉默Cav-1基因可抑制人膀胱癌细胞株5637的细胞增殖并促进细胞凋亡。“,”Objective]To explore the effect of the silencing of caveolin-1(Cav-1)gene on the proliferation and apoptosis of human bladder cancer cell line 5637 in vitro.[Methods]Cav-1 gene was transfected into human blad-der cancer cell line 5637 by siRNA(silence group).Negative control group was transfected with FAM-siRNA, while normal control group was not transfected with FAM-siRNA.Real-time PCR was used to detect the efficacy of the silencing of CAV-1 gene.CCK-8 method was used to detect the cell proliferation in each group.Cell apopto-sis was measured by flow cytometry.[Results]SiRNA transfection could significantly reduce the mRNA expres-sion of Cav-1 in human bladder cancer cell line 5637.In the silence group,cell proliferation was obviously de-creased and cell apoptosis was obviously increased(all P<0.05).[Conclusion]The silencing of Cav-1 gene can in-hibit the proliferation and promote the apoptosis of human bladder cancer cells.