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目的探讨人参皂苷Rg1热裂解产物(HPPRg1)对H_(22)荷瘤小鼠的抗肿瘤作用以及机制。方法建立ICR小鼠H_(22)皮下移植瘤小鼠模型,观察HPPRg1的抗肿瘤作用。首先将H_(22)荷瘤小鼠随机分为空白组(normal)、模型组(model)、阳性药环磷酰胺组(CTX,30 mg·kg~(-1))、HPPRg1低、中和高给药组(10、20和40 mg·kg~(-1))。通过计算抑瘤率、脏器指数和肿瘤组织切片染色来观察HPPRg1的抗肿瘤作用。结果与模型组比较,HPPRg1 3个剂量组均能剂量依赖性抑制H_(22)肿瘤的增长(r=0.99,P<0.05),抑瘤率分别为35.7%,42.9%和47.3%;此外,HPPRg1能够不同程度升高小鼠血清中肿瘤坏死因子(TNF)-α,干扰素(IFN)-γ和白细胞介素(IL)-2水平,病理组织染色表明,HPPRg1能够明显促进肿瘤细胞凋亡和坏死;其主要通过抑制瘤重细胞增殖及促凋亡而发挥抗肿瘤作用。结论 HPPRg1对H_(22)移植瘤具有明显的抑制作用,其机制可能与促进肿瘤细胞凋亡及提高机体免疫力有关。
Objective To investigate the antitumor effect and mechanism of ginsenoside Rg1 pyrolysis product (HPPRg1) on H22 tumor-bearing mice. Methods Mouse model of H22 subcutaneously transplanted in ICR mice was established and the antitumor effect of HPPRg1 was observed. H22 tumor-bearing mice were randomly divided into normal group, model group, CTX group (30 mg · kg -1), HPPRg1 low and medium High dose group (10, 20 and 40 mg · kg -1). The antitumor effect of HPPRg1 was observed by calculating the tumor inhibition rate, organ index and tumor tissue section staining. Results Compared with model group, HPPRg1 dose-dependently inhibited the growth of H22 tumors (r = 0.99, P <0.05), and the tumor inhibition rates were 35.7%, 42.9% and 47.3%, respectively. HPPRg1 could increase the level of tumor necrosis factor (TNF) -α, IFN-γ and IL-2 in mice serum to varying degrees, and the histopathological staining showed that HPPRg1 could obviously promote the apoptosis of tumor cells And necrosis; its antitumor effect is exerted mainly by inhibiting tumor cell proliferation and promoting apoptosis. Conclusion HPPRg1 has a significant inhibitory effect on H_ (22) xenografts, which may be related to the promotion of tumor cell apoptosis and the enhancement of immunity.