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目的本研究检测p16蛋白在结直肠癌组织中的缺失情况及与病理分型和临床分期的关系,探讨p16基因对结直肠癌的抑制作用。方法应用SP免疫组织化学法检测p16蛋白在32例结直肠癌患者中的表达。结果 p16基因在结直肠癌组织中的缺失率为65.6%,与非癌症的结直肠组织中缺失率为16.7%相比,差异有统计学意义(P<0.05);P16在高分化腺癌中的缺失率为40.0%,而在中分化腺癌和低分化腺癌中的缺失率为63.6%和81.8%,各组间比较差异有统计学意义(P<0.05);结直肠癌组织中p16在A、B期的缺失率为35.7%,C期的缺失率为60.0%,D期中的缺失率为62.5%。结论 p16基因在结直肠癌组织中存在着高频的缺失现象,其缺失率与肿瘤的恶性程度有关,对结直肠癌有重要的抑制作用。
Objective This study was to detect the loss of p16 protein in colorectal cancer tissues and its relationship with pathological type and clinical stage and to investigate the inhibitory effect of p16 gene on colorectal cancer. Methods SP immunohistochemistry was used to detect the expression of p16 protein in 32 patients with colorectal cancer. Results The deletion rate of p16 gene in colorectal cancer tissues was 65.6%, which was significantly lower than that in non-cancerous colorectal tissues (16.7%) (P <0.05). P16 in well-differentiated adenocarcinoma , The rate of deletion was 40.0% in the differentiated adenocarcinoma and poorly differentiated adenocarcinoma (63.6% vs 81.8%, P <0.05). There was no significant difference between the two groups The deletion rate in stage A and B was 35.7%, the rate in stage C was 60.0%, and the rate in stage D was 62.5%. Conclusion There is a high frequency of deletion of p16 gene in colorectal cancer tissues. The loss rate of p16 gene is related to the malignancy of colorectal cancer and has an important inhibitory effect on colorectal cancer.